Protein Info for Rv1640c in Mycobacterium tuberculosis H37Rv

Annotation: Lysyl-tRNA synthetase 2 LysX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1172 transmembrane" amino acids 79 to 104 (26 residues), see Phobius details amino acids 120 to 141 (22 residues), see Phobius details amino acids 147 to 168 (22 residues), see Phobius details amino acids 180 to 197 (18 residues), see Phobius details amino acids 209 to 231 (23 residues), see Phobius details amino acids 268 to 291 (24 residues), see Phobius details amino acids 613 to 630 (18 residues), see Phobius details PF16995: tRNA-synt_2_TM" amino acids 76 to 290 (215 residues), 272.8 bits, see alignment E=4.5e-85 PF09924: LPG_synthase_C" amino acids 309 to 607 (299 residues), 328 bits, see alignment E=9.7e-102 TIGR00499: lysine--tRNA ligase" amino acids 684 to 1171 (488 residues), 448.5 bits, see alignment E=1.4e-138 PF01336: tRNA_anti-codon" amino acids 726 to 804 (79 residues), 47.2 bits, see alignment 3.4e-16 PF00152: tRNA-synt_2" amino acids 821 to 1168 (348 residues), 258 bits, see alignment E=2.2e-80

Best Hits

Swiss-Prot: 100% identical to LYSX_MYCBO: Lysylphosphatidylglycerol biosynthesis bifunctional protein LysX (lysX) from Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)

KEGG orthology group: K04567, lysyl-tRNA synthetase, class II [EC: 6.1.1.6] (inferred from 100% identity to mra:MRA_1651)

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.6

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1172 amino acids)

>Rv1640c Lysyl-tRNA synthetase 2 LysX (Mycobacterium tuberculosis H37Rv)
VGLHLTVPGLRRDGRGVQSNSHDTSSKTTADISRCPQHTDAGLQRAATPGISRLLGISSR
SVTLTKPRSATRGNSRYHWVPAAAGWTVGVIATLSLLASVSPLIRWIIKVPREFINDYLF
NFPDTNFAWSFVLALLAAALTARKRIAWLVLLANMVLAAVVNAAEIAAGGNTAAESFGEN
LGFAVHVVAIVVLVLGYREFWAKVRRGALFRAAAVWLAGAVVGIVASWGLVELFPGSLAP
DERLGYAANRVVGFALADPDLFTGRPHVFLNAIFGLFGAFALIGAAIVLFLSQRADNALT
GEDESAIRGLLDLYGKDDSLGYFATRRDKSVVFASSGRACITYRVEVGVCLASGDPVGDH
RAWPQAVDAWLRLCQTYGWAPGVMGASSQGAQTYREAGLTALELGDEAILRPADFKLSGP
EMRGVRQAVTRARRAGLTVRIRRHRDIAEDEMAQTITRADSWRDTETERGFSMALGRLGD
PADSDCLLVEAIDPHNQVLAMLSLVPWGTTGVSLDLMRRSPQSPNGTIELMVSELALHAE
SLGITRISLNFAVFRAAFEQGAQLGAGPVARLWRGLLVFFSRWWQLETLYRSNMKYQPEW
VPRYACYEDARVIPRVGVASVIAEGFLVLPFSRRNRVHTGHHPAVPERLAATGLLHHDGS
APDVSGLRQVGLTNGDGVERRLPEQVRVRFDKLEKLRSSGIDAFPVGRPPSHTVAQALAA
DHQASVSVSGRIMRIRNYGGVLFAQLRDWSGEMQVLLDNSRLDQGCAADFNAATDLGDLV
EMTGHMGASKTGTPSLIVSGWRLIGKCLRPLPNKWKGLLDPEARVRTRYLDLAVNAESRA
LITARSSVLRAVRETLFAKGFVEVETPILQQLHGGATARPFVTHINTYSMDLFLRIAPEL
YLKRLCVGGVERVFELGRAFRNEGVDFSHNPEFTLLEAYQAHADYLEWIDGCRELIQNAA
QAANGAPIAMRPRTDKGSDGTRHHLEPVDISGIWPVRTVHDAISEALGERIDADTGLTTL
RKLCDAAGVPYRTQWDAGAVVLELYEHLVECRTEQPTFYIDFPTSVSPLTRPHRSKRGVA
ERWDLVAWGIELGTAYSELTDPVEQRRRLQEQSLLAAGGDPEAMELDEDFLQAMEYAMPP
TGGLGMGIDRVVMLITGRSIRETLPFPLAKPH