Protein Info for Rv1121 in Mycobacterium tuberculosis H37Rv
Annotation: Probable glucose-6-phosphate 1-dehydrogenase Zwf1 (G6PD)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to G6PD1_MYCBO: Glucose-6-phosphate 1-dehydrogenase 1 (zwf1) from Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
KEGG orthology group: K00036, glucose-6-phosphate 1-dehydrogenase [EC: 1.1.1.49] (inferred from 100% identity to mtc:MT1153)Predicted SEED Role
"Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49)" in subsystem Entner-Doudoroff Pathway or Pentose phosphate pathway (EC 1.1.1.49)
MetaCyc Pathways
- aspartate superpathway (23/25 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
- L-lysine biosynthesis I (9/9 steps found)
- pentose phosphate pathway (8/8 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
- superpathway of glucose and xylose degradation (14/17 steps found)
- superpathway of glycolysis and the Entner-Doudoroff pathway (14/17 steps found)
- L-lysine biosynthesis VI (6/7 steps found)
- pentose phosphate pathway (oxidative branch) I (3/3 steps found)
- Entner-Doudoroff pathway I (7/9 steps found)
- L-lysine biosynthesis II (7/9 steps found)
- heterolactic fermentation (13/18 steps found)
- cytosolic NADPH production (yeast) (3/5 steps found)
- formaldehyde oxidation I (3/6 steps found)
- superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (4/8 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of plant hormones
- Glutathione metabolism
- Pentose phosphate pathway
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.49
Use Curated BLAST to search for 1.1.1.49
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (466 amino acids)
>Rv1121 Probable glucose-6-phosphate 1-dehydrogenase Zwf1 (G6PD) (Mycobacterium tuberculosis H37Rv) LVDGGGGASDLLVIFGITGDLARKMTFRALYRLERHQLLDCPILGVASDDMSVGQLVKWA RESIGRTEKIDDAVFDRLAGRLSYLHGDVTDSQLYDSLAELIGSACRPLYYLEMPPALFA PIVENLANVRLLERARVAVEKPFGHDLASALELNARLRAVLGEDQILRVDHFLGKQPVVE LEYLRFANQALAELWDRNSISEIHITMAEDFGVEDRGKFYDAVGALRDVVQNHLLQVLAL VTMEPPVGSSADDLNDKKAEVFRAMAPLDPDRCVRGQYLGYTEVAGVASDSATETYVALR TEIDNWRWAGVPIFVRAGKELPAKVTEVRLFLRRVPALAFLPNRRPAEPNQIVLRIDPDP GMRLQISAHTDDSWRDIHLDSSFAVDLGEPIRPYERLLYAGLVGDHQLFAREDSIEQTWR IVQPLLDNPGEIHRYDRGSWGPEAAQSLLRGHRGWQSPWLPRGTDA