Protein Info for Rv0858c in Mycobacterium tuberculosis H37Rv

Annotation: Probable N-succinyldiaminopimelate aminotransferase DapC (DAP-at)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00155: Aminotran_1_2
  • amino acids 27 to 388 (362 residues), 214.2 bits, E=4.8e-67
• PF00266: Aminotran_5
  • amino acids 84 to 199 (116 residues), 29.7 bits, E=5e-11

Best Hits

Swiss-Prot: 100% identical to DAPC_MYCTU: Probable N-succinyldiaminopimelate aminotransferase DapC (dapC) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K14267, N-succinyldiaminopimelate aminotransferase [EC: 2.6.1.17] (inferred from 100% identity to mtu:Rv0858c)

Predicted SEED Role

"putative aminotransferase"

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• L-lysine biosynthesis I (9/9 steps found)

KEGG Metabolic Maps

• Lysine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.17

Use Curated BLAST to search for 2.6.1.17

Protein Sequence (397 amino acids)

>Rv0858c Probable N-succinyldiaminopimelate aminotransferase DapC (DAP-at) (Mycobacterium tuberculosis H37Rv)
MTVSRLRPYATTVFAEMSALATRIGAVNLGQGFPDEDGPPKMLQAAQDAIAGGVNQYPPG
PGSAPLRRAIAAQRRRHFGVDYDPETEVLVTVGATEAIAAAVLGLVEPGSEVLLIEPFYD
SYSPVVAMAGAHRVTVPLVPDGRGFALDADALRRAVTPRTRALIINSPHNPTGAVLSATE
LAAIAEIAVAANLVVITDEVYEHLVFDHARHLPLAGFDGMAERTITISSAAKMFNCTGWK
IGWACGPAELIAGVRAAKQYLSYVGGAPFQPAVALALDTEDAWVAALRNSLRARRDRLAA
GLTEIGFAVHDSYGTYFLCADPRPLGYDDSTEFCAALPEKVGVAAIPMSAFCDPAAGQAS
QQADVWNHLVRFTFCKRDDTLDEAIRRLSVLAERPAT