Protein Info for Rv0542c in Mycobacterium tuberculosis H37Rv
Annotation: Possible O-succinylbenzoic acid--CoA ligase MenE (OSB-CoA synthetase) (O-succinylbenzoate-CoA synthase)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to MENE_MYCTO: Probable 2-succinylbenzoate--CoA ligase (menE) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
KEGG orthology group: K01911, O-succinylbenzoic acid--CoA ligase [EC: 6.2.1.26] (inferred from 100% identity to mbt:JTY_0556)Predicted SEED Role
"O-succinylbenzoic acid--CoA ligase (EC 6.2.1.26)" in subsystem Menaquinone and Phylloquinone Biosynthesis (EC 6.2.1.26)
MetaCyc Pathways
- superpathway of menaquinol-7 biosynthesis (9/10 steps found)
- 2-carboxy-1,4-naphthoquinol biosynthesis (6/7 steps found)
- superpathway of menaquinol-10 biosynthesis (8/10 steps found)
- superpathway of menaquinol-11 biosynthesis (8/10 steps found)
- superpathway of menaquinol-12 biosynthesis (8/10 steps found)
- superpathway of menaquinol-13 biosynthesis (8/10 steps found)
- superpathway of menaquinol-6 biosynthesis (8/10 steps found)
- superpathway of menaquinol-8 biosynthesis I (8/10 steps found)
- superpathway of menaquinol-9 biosynthesis (8/10 steps found)
- superpathway of demethylmenaquinol-6 biosynthesis I (7/9 steps found)
- superpathway of demethylmenaquinol-8 biosynthesis I (7/9 steps found)
- superpathway of demethylmenaquinol-9 biosynthesis (7/9 steps found)
- superpathway of chorismate metabolism (40/59 steps found)
- superpathway of phylloquinol biosynthesis (7/15 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.2.1.26
Use Curated BLAST to search for 6.2.1.26
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (362 amino acids)
>Rv0542c Possible O-succinylbenzoic acid--CoA ligase MenE (OSB-CoA synthetase) (O-succinylbenzoate-CoA synthase) (Mycobacterium tuberculosis H37Rv) VLGGSDPALVAVPTQHESLLGALRVGEQIDDDVALVVTTSGTTGPPKGAMLTAAALTASA SAAHDRLGGPGSWLLAVPPYHIAGLAVLVRSVIAGSVPVELNVSAGFDVTELPNAIKRLG SGRRYTSLVAAQLAKALTDPAATAALAELDAVLIGGGPAPRPILDAAAAAGITVVRTYGM SETSGGCVYDGVPLDGVRLRVLAGGRIAIGGATLAKGYRNPVSPDPFAEPGWFHTDDLGA LESGDSGVLTVLGRADEAISTGGFTVLPQPVEAALGTHPAVRDCAVFGLADDRLGQRVVA AIVVGDGCPPPTLEALRAHVARTLDVTAAPRELHVVNVLPRRGIGKVDRAALVRRFAGEA DQ