Protein Info for Rv0346c in Mycobacterium tuberculosis H37Rv

Annotation: Possible L-asparagine permease AnsP2 (L-asparagine transport protein)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 487 transmembrane" amino acids 29 to 49 (21 residues), see Phobius details amino acids 55 to 78 (24 residues), see Phobius details amino acids 98 to 122 (25 residues), see Phobius details amino acids 136 to 157 (22 residues), see Phobius details amino acids 167 to 186 (20 residues), see Phobius details amino acids 208 to 231 (24 residues), see Phobius details amino acids 252 to 274 (23 residues), see Phobius details amino acids 295 to 316 (22 residues), see Phobius details amino acids 343 to 364 (22 residues), see Phobius details amino acids 372 to 395 (24 residues), see Phobius details amino acids 416 to 434 (19 residues), see Phobius details amino acids 440 to 460 (21 residues), see Phobius details PF00324: AA_permease" amino acids 27 to 436 (410 residues), 363.5 bits, see alignment E=1.6e-112 PF13520: AA_permease_2" amino acids 28 to 435 (408 residues), 124 bits, see alignment E=7.7e-40

Best Hits

Swiss-Prot: 100% identical to ANSP2_MYCTO: L-asparagine permease 2 (ansP2) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

KEGG orthology group: K11738, L-asparagine permease (inferred from 100% identity to mtb:TBMG_00350)

MetaCyc: 35% identical to D-serine/alanine/glycine:H+symporter (Escherichia coli K-12 substr. MG1655)
RXN0-5130; RXN0-5201; RXN0-5202; RXN0-5203; TRANS-RXN-62A; TRANS-RXN-62B

Predicted SEED Role

"L-asparagine permease"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (487 amino acids)

>Rv0346c Possible L-asparagine permease AnsP2 (L-asparagine transport protein) (Mycobacterium tuberculosis H37Rv)
MPPLDITDERLTREDTGYHKGLHSRQLQMIALGGAIGTGLFLGAGGRLASAGPGLFLVYG
ICGIFVFLILRALGELVLHRPSSGSFVSYAREFYGEKVAFVAGWMYFLNWAMTGIVDTTA
IAHYCHYWRAFQPIPQWTLALIALLVVLSMNLISVRLFGELEFWASLIKVIALVTFLIVG
TVFLAGRYKIDGQETGVSLWSSHGGIVPTGLLPIVLVTSGVVFAYAAIELVGIAAGETAE
PAKIMPRAINSVVLRIACFYVGSTVLLALLLPYTAYKEHVSPFVTFFSKIGIDAAGSVMN
LVVLTAALSSLNAGLYSTGRILRSMAINGSGPRFTAPMSKTGVPYGGILLTAGIGLLGII
LNAIKPSQAFEIVLHIAATGVIAAWATIVACQLRLHRMANAGQLQRPKFRMPLSPFSGYL
TLAFLAGVLILMYFDEQHGPWMIAATVIGVPALIGGWYLVRNRVTAVAHHAIDHTKSVAV
VHSADPI