Protein Info for Rv0003 in Mycobacterium tuberculosis H37Rv

Annotation: DNA replication and repair protein RecF (single-strand DNA binding protein)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 385 TIGR00611: DNA replication and repair protein RecF" amino acids 1 to 379 (379 residues), 447.5 bits, see alignment E=1.9e-138 PF02463: SMC_N" amino acids 2 to 353 (352 residues), 62.3 bits, see alignment E=4.6e-21 PF13476: AAA_23" amino acids 6 to 59 (54 residues), 34.7 bits, see alignment 2.8e-12

Best Hits

Swiss-Prot: 100% identical to RECF_MYCTU: DNA replication and repair protein RecF (recF) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K03629, DNA replication and repair protein RecF (inferred from 99% identity to mbb:BCG_0003)

Predicted SEED Role

"DNA recombination and repair protein RecF" in subsystem DNA repair, bacterial RecFOR pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (385 amino acids)

>Rv0003 DNA replication and repair protein RecF (single-strand DNA binding protein) (Mycobacterium tuberculosis H37Rv)
VYVRHLGLRDFRSWACVDLELHPGRTVFVGPNGYGKTNLIEALWYSTTLGSHRVSADLPL
IRVGTDRAVISTIVVNDGRECAVDLEIATGRVNKARLNRSSVRSTRDVVGVLRAVLFAPE
DLGLVRGDPADRRRYLDDLAIVRRPAIAAVRAEYERVLRQRTALLKSVPGARYRGDRGVF
DTLEVWDSRLAEHGAELVAARIDLVNQLAPEVKKAYQLLAPESRSASIGYRASMDVTGPS
EQSDIDRQLLAARLLAALAARRDAELERGVCLVGPHRDDLILRLGDQPAKGFASHGEAWS
LAVALRLAAYQLLRVDGGEPVLLLDDVFAELDVMRRRALATAAESAEQVLVTAAVLEDIP
AGWDARRVHIDVRADDTGSMSVVLP