Protein Info for RS_RS20525 in Ralstonia solanacearum GMI1000

Annotation: cytochrome P450

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 398 PF00067: p450" amino acids 82 to 368 (287 residues), 105 bits, see alignment E=2.1e-34

Best Hits

Swiss-Prot: 78% identical to C1332_XYLFT: Putative cytochrome P450 133B2 (cyp133B2) from Xylella fastidiosa (strain Temecula1 / ATCC 700964)

KEGG orthology group: K00517, [EC: 1.14.-.-] (inferred from 100% identity to rso:RS01740)

Predicted SEED Role

"putative cytochrome P450 hydroxylase" in subsystem Nitric oxide synthase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.-.-

Use Curated BLAST to search for 1.14.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XRX2 at UniProt or InterPro

Protein Sequence (398 amino acids)

>RS_RS20525 cytochrome P450 (Ralstonia solanacearum GMI1000)
MKLADLSTPSFLENPYPLYETLRSQGPFVRIGPNALMTGHYSIVDALLHNRQMGKSYMES
IRLRYGDEGPNMPLFQGFSRMFLMLNPPMHTRLRGLMMQVFNARQIESMREVATATAHQL
IDDFEQKPSADLVAEFAFPLPVRIICQMMDLDIDDAMALGVGVSKLAKVFDPAPMSADAL
VETSAAYEELAQYFTKVIEARRAQPGTDLISMLMRAEENGETLTHDEIVSNVILLFIAGH
ETTSNMIGNALIALHRNPQQLDLLKREPSRMPNAVLECLRYDGSVQVTIRAALEDVEVEG
EVLPRGTTVFLMLGAANRDPAQFTDPDQLDIGRQQGRLQTFGAGIHHCLGYRLALIELES
ALGALFERLPNLRLTNLDQLSWNQRGNLRGVNALMAAW