Protein Info for RS_RS16675 in Ralstonia solanacearum GMI1000

Annotation: tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 647 TIGR00136: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA" amino acids 7 to 631 (625 residues), 901.3 bits, see alignment E=1.4e-275 PF12831: FAD_oxidored" amino acids 8 to 160 (153 residues), 34.5 bits, see alignment E=3.8e-12 PF01134: GIDA" amino acids 8 to 399 (392 residues), 564 bits, see alignment E=4.8e-173 PF21680: GIDA_C_1st" amino acids 461 to 565 (105 residues), 84.9 bits, see alignment E=1.3e-27 PF13932: GIDA_C" amino acids 571 to 626 (56 residues), 89.8 bits, see alignment 2e-29

Best Hits

Swiss-Prot: 100% identical to MNMG_RALSO: tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K03495, glucose inhibited division protein A (inferred from 100% identity to rso:RSc3328)

MetaCyc: 73% identical to 5-carboxymethylaminomethyluridine-tRNA synthase subunit MnmG (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XU65 at UniProt or InterPro

Protein Sequence (647 amino acids)

>RS_RS16675 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG (Ralstonia solanacearum GMI1000)
MLYPVEFDVIVVGGGHAGTEAALAAARMGCQTLLLTHNIETLGQMSCNPSIGGIGKGHLV
KEVDALGGAMALATDEGGIQFRILNSSKGPAVRATRAQADRVLYKAAIRHRLENQPNLML
FQQAVEDLLVEGDRVVGAVTQVGVRFRARAVVLTAGTFLDGKIHVGLNNYTGGRAGDPAA
VSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSVLEAQPGDLDPVPVFSFLGRTDMHPRQ
VPCWVTHTNERTHDIIRAGLNRSPMYTGVIEGVGPRYCPSIEDKIHRFASKDSHQIFLEP
EGLTTNEFYPNGVSTSLPFDVQLDLIHSMRGLEHAHILRPGYAIEYDYFDPRGLKASLES
KAIGGLFFAGQINGTTGYEEAAAQGLLAGINAGLQVQGKAAWTPRRDQAYLGVLVDDLIT
RGVTEPYRMFTSRAEFRLSLREDNADMRLTEAGRDLGVVDDARWDAFNRKRDAVSRETER
LKSTWVNPAILPAADAEPVLGKGIEREYSLADLLRRPNVTYESLMGMQGGKYAPDAPLAD
EPLLAEQIREQIEIGIKYHGYIARQADEVERLGANENTRLPADFDYKQVRGLSIEVQQKL
AQHKPETIGQASRISGITPAAVSLLLVHLKKGVLRGKVGPRSEGEAA