Protein Info for RS_RS13255 in Ralstonia solanacearum GMI1000

Annotation: urocanate hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF17391: Urocanase_N
  • amino acids 22 to 148 (127 residues), 205.9 bits, E=2.3e-65
• PF01175: Urocanase
  • amino acids 151 to 174 (24 residues), 23.6 bits, see alignment (E = 5.5e-09)
  • amino acids 198 to 394 (197 residues), 232.4 bits, E=6.9e-73
• TIGR01228: urocanate hydratase
  • amino acids 201 to 599 (399 residues), 643.6 bits, E=1.3e-197
• PF17392: Urocanase_C
  • amino acids 397 to 591 (195 residues), 312.6 bits, E=1.4e-97

Best Hits

Swiss-Prot: 100% identical to HUTU_RALSO: Urocanate hydratase (hutU) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01712, urocanate hydratase [EC: 4.2.1.49] (inferred from 100% identity to rso:RSc2647)

Predicted SEED Role

"Urocanate hydratase (EC 4.2.1.49)" in subsystem Histidine Degradation (EC 4.2.1.49)

MetaCyc Pathways

• L-histidine degradation VI (7/8 steps found)
• L-histidine degradation I (4/4 steps found)
• L-histidine degradation II (4/5 steps found)
• L-histidine degradation III (4/6 steps found)

KEGG Metabolic Maps

• Histidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.49

Sequence Analysis Tools

See Q8XW28 at UniProt or InterPro

Protein Sequence (601 amino acids)

>RS_RS13255 urocanate hydratase (Ralstonia solanacearum GMI1000)
MNTMTDAAHLAADPRYDAAREIRAPRGTELHCKSWLTEAAYRMLQNNLDPDVAENPKHLV
VYGGIGRAARDWACFDKILETLRELNDDESLLVQSGKPVGVFKTHPDAPRVLIANSNLVP
KWANWEHFNALDRKGLFMYGQMTAGSWIYIGSQGIVQGTYETFAEAGRQHYSDRPSALLK
QGLSPEGTAPGSGRSSAQVPGSHLAGRWILTAGLGGMGGAQPLAATLAGAVSLTIECQQS
SIDFRLRTRYLDKQARDIDDALNLIRHHCERGEAVSIGLLGNAAELLPELVRRARAGGLK
PDLVTDQTSAHDLVNGYLPAGWTVEQWRAAQRDPAQHAHLSAEAARSCAVHVQAMLDFQS
MGIPTVDYGNNIRQVAFDQGVKNAFDFPGFVPAYIRPLFCEGRGPFRWVALSGDPEDIYK
TDAKIKELFPHNAHVHRWLDMARERIAFQGLPARICWLGLGERHAAGLAFNEMVRKGELK
APIVIGRDHLDTGSVASPNRETEAMRDGTDAVSDWPLLNALLNTAGGATWVSLHHGGGVG
MGYSQHAGVVIVADGTDAAARRLARVLVNDAGSGVMRHADAGYETAVACAKRNGLKLPMI
G