Protein Info for RS_RS03390 in Ralstonia solanacearum GMI1000

Annotation: 16S rRNA pseudouridine(516) synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 252 PF01479: S4" amino acids 13 to 49 (37 residues), 24.9 bits, see alignment 1.4e-09 PF00849: PseudoU_synth_2" amino acids 69 to 200 (132 residues), 62.9 bits, see alignment E=4.3e-21 TIGR00093: pseudouridine synthase" amino acids 73 to 231 (159 residues), 135.2 bits, see alignment E=8.7e-44

Best Hits

KEGG orthology group: K06183, ribosomal small subunit pseudouridine synthase A [EC: 5.4.99.12] (inferred from 100% identity to rso:RSc0674)

Predicted SEED Role

"Ribosomal small subunit pseudouridine synthase A (EC 4.2.1.70)" (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8Y1L6 at UniProt or InterPro

Protein Sequence (252 amino acids)

>RS_RS03390 16S rRNA pseudouridine(516) synthase (Ralstonia solanacearum GMI1000)
MTASSARLPLYRILQSQGFGTRRYCKDLVFAGLVHVNGVEAEDPDAQVDTAGLVLDVDGE
RWAYHARAYLMLHKPTGFECSQKPRHHPSVYTLLPVPLRERGVQCVGRLDQDTTGLLLLT
DDGQFVHTLTSPRHQVPKVYEITTAEPVTDAQVTQLCQGVLLEDEDVPVAAAWAERADAC
HLRMALTQGKYHQVKRMVAAAGNHVAALHRSAIGGLTLGADLAPGQWRWLESDDLVALDT
TPVHPAGAAMGA