Protein Info for RS_RS01120 in Ralstonia solanacearum GMI1000

Annotation: RNA 3'-terminal phosphate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 347 TIGR03399: RNA 3'-phosphate cyclase" amino acids 11 to 331 (321 residues), 386.4 bits, see alignment E=5.3e-120 PF01137: RTC" amino acids 18 to 333 (316 residues), 275.8 bits, see alignment E=2e-86 PF05189: RTC_insert" amino acids 190 to 281 (92 residues), 51.1 bits, see alignment E=1.5e-17

Best Hits

Swiss-Prot: 100% identical to RTCA_RALSO: RNA 3'-terminal phosphate cyclase (rtcA) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01974, RNA 3'-terminal phosphate cyclase [EC: 6.5.1.4] (inferred from 100% identity to rso:RSc0226)

Predicted SEED Role

"RNA 3'-terminal phosphate cyclase (EC 6.5.1.4)" in subsystem RNA 3'-terminal phosphate cyclase (EC 6.5.1.4)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8Y2V6 at UniProt or InterPro

Protein Sequence (347 amino acids)

>RS_RS01120 RNA 3'-terminal phosphate cyclase (Ralstonia solanacearum GMI1000)
MNNRLQPAPCIELDGAQGEGGGQILRTALTLSMLTGTPFRIERIRAGRSKPGLMRQHLTA
VQAAAEVSGATVEGAEAGSQALAFAPGPIHGGDYRFAIGTAGSCTLVLQTVLPALWFADA
PSTVAVSGGTHNRAAPPVDFLIRAWQPLLARMGVTQTLALKRHGFYPAGGGEVLATVTPC
AGRLGALHLTERGALRELSGQGIVANVRPGVARRELEALAARVPGVVGSVRELSPAEGPG
NALVLDAVHEHVTEVFTGFGERGVPAEQVAHGVASAALRYLHSTAAVDEYLADQLVLPMA
LAGAGCFTAVTASPHLTTNIAVIEKFLPVHITVQAERDASVVRVQVN