Protein Info for RS_RS00270 in Ralstonia solanacearum GMI1000
Annotation: Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit B
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to GATB_RALSO: Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B (gatB) from Ralstonia solanacearum (strain GMI1000)
KEGG orthology group: K02434, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit B [EC: 6.3.5.6 6.3.5.7] (inferred from 100% identity to rso:RSc0054)MetaCyc: 50% identical to glutamyl-tRNA(Gln) amidotransferase, B subunit (Synechococcus elongatus PCC 7942 = FACHB-805)
Glutaminyl-tRNA synthase (glutamine-hydrolyzing). [EC: 6.3.5.7]; Asparaginyl-tRNA synthase (glutamine-hydrolyzing). [EC: 6.3.5.7, 6.3.5.6]
Predicted SEED Role
"Aspartyl-tRNA(Asn) amidotransferase subunit B (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit B (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)
MetaCyc Pathways
- L-citrulline biosynthesis (8/8 steps found)
- L-glutamate and L-glutamine biosynthesis (7/7 steps found)
- superpathway of L-citrulline metabolism (10/12 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7
Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8Y3C6 at UniProt or InterPro
Protein Sequence (488 amino acids)
>RS_RS00270 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit B (Ralstonia solanacearum GMI1000) MQWEVVIGLETHAQLSTASKIFSGASTAFGAAPNTQAAPVDLALPGVLPVLNKGAVERAI TFGLAIGAKIASKSIFARKNYFYPDLPKGYQISQYEIPVVQGGTLTIQVEGRNGQPGYEK TVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPDMRSAAEAVAYARALHA LVMWLGICDGNMQEGSFRCDANVSVRRGPDAPFGTRCEIKNLNSFRFLEEAINYEVRRQI ELIEDGGTVVQETRLYDPDRKETRSMRSKEDAHDYRYFPDPDLLPLMIGADWVDAVRATL PELPAAMAARFESAYGLPRYDASILTATKATAAYFEAVVAAAGAANAKAAANWIMGEVAS NLNRAGLEIDAAPVSPVQLAGLLARIADGTISNNTAKKDVFPAMWAGEHGGDADAIIAEK GLKQMSDSGELEKIIDEVLAANAKSVEEFRAGKDKAFNALVGQAMKATRGKANPSQVNEL LKKKLGAA