Protein Info for RR42_RS36505 in Cupriavidus basilensis FW507-4G11

Annotation: long-chain fatty acid--CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 38 to 422 (385 residues), 293.6 bits, E=2.1e-91
• PF13193: AMP-binding_C
  • amino acids 476 to 555 (80 residues), 77.6 bits, E=1.2e-25

Best Hits

KEGG orthology group: K01897, long-chain acyl-CoA synthetase [EC: 6.2.1.3] (inferred from 79% identity to reu:Reut_B3549)

Predicted SEED Role

No annotation

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (50/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• oleate β-oxidation (29/35 steps found)
• fatty acid salvage (5/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• octane oxidation (4/5 steps found)
• long-chain fatty acid activation (1/1 steps found)
• palmitate biosynthesis III (21/29 steps found)
• phytol degradation (3/4 steps found)
• stearate biosynthesis IV (4/6 steps found)
• γ-linolenate biosynthesis II (animals) (1/2 steps found)
• linoleate biosynthesis II (animals) (1/2 steps found)
• alkane biosynthesis II (1/3 steps found)
• oleate biosynthesis I (plants) (1/3 steps found)
• 3-methyl-branched fatty acid α-oxidation (3/6 steps found)
• 6-gingerol analog biosynthesis (engineered) (3/6 steps found)
• long chain fatty acid ester synthesis (engineered) (1/4 steps found)
• phosphatidylcholine acyl editing (1/4 steps found)
• wax esters biosynthesis II (1/4 steps found)
• stearate biosynthesis I (animals) (2/6 steps found)
• ceramide degradation by α-oxidation (2/7 steps found)
• sphingosine and sphingosine-1-phosphate metabolism (4/10 steps found)
• arachidonate biosynthesis III (6-desaturase, mammals) (1/7 steps found)
• capsaicin biosynthesis (1/7 steps found)
• icosapentaenoate biosynthesis II (6-desaturase, mammals) (1/7 steps found)
• icosapentaenoate biosynthesis III (8-desaturase, mammals) (1/7 steps found)
• sporopollenin precursors biosynthesis (8/18 steps found)
• ceramide and sphingolipid recycling and degradation (yeast) (4/16 steps found)
• cutin biosynthesis (3/16 steps found)
• suberin monomers biosynthesis (5/20 steps found)

KEGG Metabolic Maps

• Fatty acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.3

Use Curated BLAST to search for 6.2.1.3

Sequence Analysis Tools

See A0A0C4YWI5 at UniProt or InterPro

Protein Sequence (580 amino acids)

>RR42_RS36505 long-chain fatty acid--CoA ligase (Cupriavidus basilensis FW507-4G11)
MNQAASATHAPAYRSPHWPAGMPAEVCVPRTSLAYNVEVAARRYPDKTAIQYFHTAISYA
GLHAQIERMAGYLQQACGVAPGDRVVLFSQNCPQFIVAYYAILRAEAVVVPANPMWLTGE
LEHVAADSGAVAAFVAAELYPQIAPLHGASLAHVIVHQYGDMLEPAGGTGGVPAWLCERA
PLPAGAPAGATLATWTQADAAGHAPRAHEAGYDTLCMLAYTSGTTGHPKGCMHTHGTLMS
AAVGSQLWRSASPQSVCLSVAPMFHLLGMQNGIHAPLYLGATIVLLPRWDRAAAADLIER
YQVSTWAAPPAMLVDFFAQPGIGERNLSSLAYLGGGGAAMPDAVATMLQERFGLPYVEAY
GLTETAAFLLSNPRHRPKRECLGIATFGVDARVIDPHTLAELPQGELGEIVTHGAQVMRG
YWRNPQADAESFIEIDGKRFLRTGDLGFMDEEGYFFMRDRLKRMINASGFKVWPAEVENL
LYGHPAIHEACVIAARDARRGETVKAVVALKPGARGTPEASAEQIMAWCRERLAAYKVPR
IVSFVDALPKSATGKIQWRALQELEQDQGGDQPAAAGSVA