Protein Info for RR42_RS35915 in Cupriavidus basilensis FW507-4G11

Annotation: beta-ketoadipyl CoA thiolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02430: 3-oxoadipyl-CoA thiolase
  • amino acids 3 to 400 (398 residues), 726.2 bits, E=1e-222
• PF00108: Thiolase_N
  • amino acids 5 to 267 (263 residues), 232.6 bits, E=8.2e-73
• TIGR01930: acetyl-CoA C-acyltransferase
  • amino acids 6 to 398 (393 residues), 446.2 bits, E=9.8e-138
• PF00109: ketoacyl-synt
  • amino acids 89 to 137 (49 residues), 21.2 bits, 3e-08
• PF02803: Thiolase_C
  • amino acids 275 to 399 (125 residues), 155 bits, E=1.1e-49

Best Hits

Swiss-Prot: 70% identical to PCAF_PSEAE: Beta-ketoadipyl-CoA thiolase (pcaF) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00632, acetyl-CoA acyltransferase [EC: 2.3.1.16] (inferred from 94% identity to cti:RALTA_B0208)

MetaCyc: 94% identical to 3-oxoadipyl-CoA thiolase (Cupriavidus necator H16)
3-oxoadipyl-CoA thiolase. [EC: 2.3.1.174]

Predicted SEED Role

"Acetyl-CoA acetyltransferase (EC 2.3.1.9) @ Beta-ketoadipyl CoA thiolase (EC 2.3.1.-)" (EC 2.3.1.-, EC 2.3.1.9)

MetaCyc Pathways

• oleate β-oxidation (29/35 steps found)
• aromatic compounds degradation via β-ketoadipate (9/9 steps found)
• mandelate degradation to acetyl-CoA (15/18 steps found)
• superpathway of salicylate degradation (7/7 steps found)
• catechol degradation III (ortho-cleavage pathway) (6/6 steps found)
• adipate biosynthesis (5/5 steps found)
• adipate degradation (5/5 steps found)
• superpathway of phenylethylamine degradation (9/11 steps found)
• benzoyl-CoA degradation I (aerobic) (6/7 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• ketolysis (3/3 steps found)
• polyhydroxybutanoate biosynthesis (3/3 steps found)
• fatty acid salvage (5/6 steps found)
• 3-oxoadipate degradation (2/2 steps found)
• acetoacetate degradation (to acetyl CoA) (2/2 steps found)
• phenylacetate degradation I (aerobic) (7/9 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (4/5 steps found)
• ketogenesis (4/5 steps found)
• 4-methylcatechol degradation (ortho cleavage) (5/7 steps found)
• acetyl-CoA fermentation to butanoate (5/7 steps found)
• fatty acid β-oxidation I (generic) (5/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (10/14 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (25/35 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (7/10 steps found)
• L-isoleucine degradation I (4/6 steps found)
• propanoate fermentation to 2-methylbutanoate (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• valproate β-oxidation (6/9 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (3/5 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• pyruvate fermentation to acetone (3/5 steps found)
• 2-deoxy-D-ribose degradation II (5/8 steps found)
• L-tryptophan degradation III (eukaryotic) (10/15 steps found)
• (2S)-ethylmalonyl-CoA biosynthesis (2/4 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
• pyruvate fermentation to butanoate (4/7 steps found)
• 3-phenylpropanoate degradation (6/10 steps found)
• 5,6-dehydrokavain biosynthesis (engineered) (6/10 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
• ethylbenzene degradation (anaerobic) (2/5 steps found)
• isopropanol biosynthesis (engineered) (2/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (17/26 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• androstenedione degradation I (aerobic) (16/25 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (10/17 steps found)
• 4-ethylphenol degradation (anaerobic) (2/6 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (7/13 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (4/9 steps found)
• glycerol degradation to butanol (9/16 steps found)
• superpathway of aerobic toluene degradation (19/30 steps found)
• fatty acid β-oxidation VII (yeast peroxisome) (1/5 steps found)
• pyruvate fermentation to butanol I (3/8 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• superpathway of testosterone and androsterone degradation (17/28 steps found)
• 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (4/10 steps found)
• superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) (4/10 steps found)
• 10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast) (1/6 steps found)
• 2-methylpropene degradation (2/8 steps found)
• ethylmalonyl-CoA pathway (4/11 steps found)
• mevalonate pathway I (eukaryotes and bacteria) (1/7 steps found)
• mevalonate pathway II (haloarchaea) (1/7 steps found)
• L-lysine fermentation to acetate and butanoate (3/10 steps found)
• methyl tert-butyl ether degradation (3/10 steps found)
• 4-oxopentanoate degradation (2/9 steps found)
• L-glutamate degradation VII (to butanoate) (4/12 steps found)
• isoprene biosynthesis II (engineered) (1/8 steps found)
• mevalonate pathway III (Thermoplasma) (1/8 steps found)
• mevalonate pathway IV (archaea) (1/8 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (7/17 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (6/16 steps found)
• 10-cis-heptadecenoyl-CoA degradation (yeast) (2/12 steps found)
• 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (2/12 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• toluene degradation VI (anaerobic) (5/18 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• cholesterol degradation to androstenedione I (cholesterol oxidase) (4/17 steps found)
• jasmonic acid biosynthesis (5/19 steps found)
• androstenedione degradation II (anaerobic) (10/27 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (20/42 steps found)
• sitosterol degradation to androstenedione (2/18 steps found)
• platensimycin biosynthesis (7/26 steps found)
• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (4/22 steps found)
• superpathway of ergosterol biosynthesis I (5/26 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (20/47 steps found)
• superpathway of L-lysine degradation (12/43 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)
• superpathway of cholesterol biosynthesis (5/38 steps found)
• superpathway of cholesterol degradation III (oxidase) (12/49 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Anthocyanin biosynthesis
• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Biosynthesis of type II polyketide backbone
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Carotenoid biosynthesis - General
• Diterpenoid biosynthesis
• Ether lipid metabolism
• Ethylbenzene degradation
• Fatty acid biosynthesis
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Glycerophospholipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• Histidine metabolism
• Limonene and pinene degradation
• Lipopolysaccharide biosynthesis
• Lysine degradation
• Phenylalanine metabolism
• Propanoate metabolism
• Pyruvate metabolism
• Synthesis and degradation of ketone bodies
• Terpenoid biosynthesis
• Tryptophan metabolism
• Tyrosine metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-, 2.3.1.16, 2.3.1.9

Use Curated BLAST to search for 2.3.1.- or 2.3.1.16 or 2.3.1.174 or 2.3.1.9

Sequence Analysis Tools

See A0A0C4YS41 at UniProt or InterPro

Protein Sequence (400 amino acids)

>RR42_RS35915 beta-ketoadipyl CoA thiolase (Cupriavidus basilensis FW507-4G11)
MTEAFICDAIRTPIGRYAGSLSGVRPDDLGAVPLKALMARNPNLDWSAIDDVIYGNANQA
GEDNRNVARMSLLLAGLPQSVPGATINRLCGSGMDATGTAARAIKSGEAGLMLAGGVESM
SRAPFVMGKATSAFSRDAQIFDTTIGWRFINPAMRAAYGVDSMPETAENVATDYKISRED
QDLMALRSQEKASRAQADGTLAQEITAVTIAQKKGDPLVVERDEHPRATSMEALGKLRGV
VRADGTVTAGNASGVNDGACAILLASEAAARQHGLTPKARIVGMATAGVAPRVMGIGPAP
ASQKLLKQLGMTIDQMDVIELNEAFAAQGLAVLRELGVADDDARVNPNGGAIALGHPLGM
SGARLVTTAMYQLHRTGGRYALCTMCIGVGQGIAMVIERV