Protein Info for RR42_RS34245 in Cupriavidus basilensis FW507-4G11

Updated annotation (from data): branched-chain alpha-ketoacid:ferredoxin oxidoreductase, fused
Rationale: Specifically important for utilizing L-Leucine as a carbon or nitrogen source.
Original annotation: indolepyruvate ferredoxin oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1186 transmembrane" amino acids 745 to 765 (21 residues), see Phobius details PF01558: POR" amino acids 752 to 937 (186 residues), 57.8 bits, see alignment E=1.6e-19 PF20169: DUF6537" amino acids 967 to 1170 (204 residues), 228.3 bits, see alignment E=9.4e-72

Best Hits

Predicted SEED Role

"Indolepyruvate ferredoxin oxidoreductase, alpha and beta subunits" in subsystem Aromatic amino acid interconversions with aryl acids

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YQW5 at UniProt or InterPro

Protein Sequence (1186 amino acids)

>RR42_RS34245 branched-chain alpha-ketoacid:ferredoxin oxidoreductase, fused (Cupriavidus basilensis FW507-4G11)
MNAPLSSAQQDALASVSLDDKYTLEKGRVYLSGTQALVRLPMLQKARDLAAGLNTAGFIS
GYRGSPLGGVDQALWKAKKHLAASDVVFQPGVNEDLAATAVWGTQQVNLFPDATRDGVFS
MWYGKGPGVDRSIDVLKHANSAGSAKHGGVLLLAGDDHAAKSSSVAHQSEHVLLASGIPV
LYPSNVQEYLDYGLHGWAMSRYSGLWVSMKCVTDVVESTASVEVDPDRVRIVLPEDFAMP
EGGLNIRWPDPPLAQEARLLDHKWYAALAYIRANKLNRVVLDSPNARFGIMTAGKAYLDV
RQALSDLGLDDDTCRRIGIRVFKVGCVWPLDAHDAREFATGLEEILVVEEKRQILEYALK
EELYNWRDDVRPKVYGKFDERGNHGGEWSLPRGNWLLPAHYELSPALIAKAIATRLEKSD
LPTDVRERIAARVALIEAKEREAARPRISVERKPWFCSGCPHNTSTRVPEGSRALAGIGC
HYMAMWMDRNTDTFSQMGGEGVAWTGQMHFTGEKHVFVNLGDGTYFHSGLLAVRASIAAK
ANITYKILFNDAVAMTGGQPVDGVLTVPQIAHQVLAEGASKIVVVTDEPEKYGDGGMLPA
SVTVHHRDQLDEIQVQLRDTAGVTILIYDQTCATEKRRRRKRGTMADPAKRAFINDAVCE
GCGDCSVKSNCLSVEPLETPLGTKRKINQSSCNKDFSCVNGFCPSFVTAEGAQVRKPAAA
GGKGALADFSALPQPALPTLERPYGVLVTGVGGTGVVTIGGLLGMAAHLEQKGVTVLDMA
GLAQKGGAVISHVQIAPTPAALHATRIATGEARLVIGCDAIVSASPEVLSKTRIDVTAAA
INSADTPTADFIKNPNWKFPGASAEQDLRASVGDACAFIDANAWAVKLLADSIYSNPLLL
GFAWQKGWVPLRRESLVRAIELNGVAVEKNLLAFDWGRYLAHHGEAALAAQLQITPRAQV
VAMPETLDSVIRQREALLTAYQNPAYAARYRAAVESVRAAEKRVGANPRLPLAEAVARNL
AKLMAYKDEYEVARLYADPAFLDKLRAQFEGEPGRDYQLSFHLAPPLLAKRDSHGHLVKR
RFGPAMLTAFRLLARAKGLRGTAFDVFGKTAERRAERKLIEDYIAMAEEFGATLNADRLD
TAVALASLPDDIRGFGHVKEANMEKAAARRVALLAQYRGAAARVAA