Protein Info for RR42_RS32225 in Cupriavidus basilensis FW507-4G11

Annotation: NAD-dependent dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 333 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF05368: NmrA" amino acids 6 to 123 (118 residues), 51.3 bits, see alignment E=5.5e-17 PF02719: Polysacc_synt_2" amino acids 7 to 139 (133 residues), 39.4 bits, see alignment E=1.8e-13 TIGR03466: hopanoid-associated sugar epimerase" amino acids 7 to 332 (326 residues), 480.4 bits, see alignment E=1.1e-148 PF04321: RmlD_sub_bind" amino acids 7 to 160 (154 residues), 28.3 bits, see alignment E=3.7e-10 PF01370: Epimerase" amino acids 7 to 234 (228 residues), 125.1 bits, see alignment E=1.2e-39 PF16363: GDP_Man_Dehyd" amino acids 8 to 244 (237 residues), 58.3 bits, see alignment E=3.7e-19 PF01073: 3Beta_HSD" amino acids 8 to 222 (215 residues), 74.7 bits, see alignment E=2.7e-24 PF13460: NAD_binding_10" amino acids 11 to 174 (164 residues), 61.7 bits, see alignment E=3.6e-20 PF07993: NAD_binding_4" amino acids 62 to 212 (151 residues), 47.3 bits, see alignment E=6.3e-16

Best Hits

KEGG orthology group: K00091, dihydroflavonol-4-reductase [EC: 1.1.1.219] (inferred from 80% identity to reh:H16_B2168)

Predicted SEED Role

"Dihydroflavonol-4-reductase (EC 1.1.1.219)" in subsystem Biflavanoid biosynthesis or Tannin biosynthesis (EC 1.1.1.219)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.219

Use Curated BLAST to search for 1.1.1.219

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YE42 at UniProt or InterPro

Protein Sequence (333 amino acids)

>RR42_RS32225 NAD-dependent dehydratase (Cupriavidus basilensis FW507-4G11)
MSSGDYVLVTGASGFLGSAVARQALARGFRVRAMVRAGSPRRNLEGLPVEIAEGDMRDAR
AMESALRGVRYLFHVAADYRLWAPDPEEIVRTNVAGTETVMGAAQACGVERIVYTSSVAT
LRVAGAAAPVTEEAAMAGHEAIGAYKRSKVLAERVVERMVARDGLPAVIVNPSTPIGPRD
VRPTPTGRIIVEAATGKIPAFVDTGLNLVHVDDVAEGHMLALARGVTGERYILGGQDVGL
QQMLADIAAMTGRRAPTMRLPRWPLYPLAYAAEAVARMTGKEPFLTADGLSMSKYRMFFS
SEKARLALGYQARPYQQGLSEALDWFRSAGYLG