Protein Info for RR42_RS32055 in Cupriavidus basilensis FW507-4G11

Updated annotation (from data): 3-carboxymuconate cycloisomerase PcaB (EC 5.5.1.2)
Rationale: Specifically important for 4-hydroxybenzoate utilization.
Original annotation: 3-carboxy-cis,cis-muconate cycloisomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02426: 3-carboxy-cis,cis-muconate cycloisomerase
  • amino acids 2 to 340 (339 residues), 422.9 bits, E=3.9e-131
• PF00206: Lyase_1
  • amino acids 8 to 290 (283 residues), 189.2 bits, E=1.2e-59
• PF10397: ADSL_C
  • amino acids 356 to 437 (82 residues), 49.6 bits, E=4.1e-17

Best Hits

Swiss-Prot: 56% identical to PCAB_PSEAE: 3-carboxy-cis,cis-muconate cycloisomerase (pcaB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01857, 3-carboxy-cis,cis-muconate cycloisomerase [EC: 5.5.1.2] (inferred from 79% identity to reh:H16_B2289)

Predicted SEED Role

"3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 5.5.1.2)

MetaCyc Pathways

• aromatic compounds degradation via β-ketoadipate (9/9 steps found)
• protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (25/35 steps found)
• toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
• superpathway of aerobic toluene degradation (19/30 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation

Isozymes

Compare fitness of predicted isozymes for: 5.5.1.2

Use Curated BLAST to search for 5.5.1.2

Sequence Analysis Tools

See A0A0C4YE08 at UniProt or InterPro

Protein Sequence (442 amino acids)

>RR42_RS32055 3-carboxymuconate cycloisomerase PcaB (EC 5.5.1.2) (Cupriavidus basilensis FW507-4G11)
MFGSAATLAAFSDTATVQRMLDFEAALARAEASCGVIPADAAEAIAATCRAGEIDFDALA
AAAVAGGNLAIPLVRQLTARVAARDAQAARYVHWGATSQDAIDTGMVLQLREALQAVDAD
LKALGHACAALAAQHRDTPMVARTWLQHALPTTFGLKAAGWLDALRRDLRRLDAARAQAA
TLQFGGAAGTLASLGAAAPAVATALGSALSLAVAPTPWHAYRDRMVEVATTLGMLTGSLG
KIARDVSLMMQTEVAEVAEPTGPGRGGSSTMPHKRNPVGCAAVLTAAVRVPPLVATMLAG
MVQEHERALGGWQAEWDTLPQIVTLAAGALRQMLEVVGGLQVDAARMRANLGVTHGLILA
EAAMLELGGKIGRLPAHHLVEGACRRAVAEGTTLRQALGATLAEDAAHAGLMDAAALDRV
CDPANYAGQAAGFVDAVLAAWR