Protein Info for RR42_RS31800 in Cupriavidus basilensis FW507-4G11

Updated annotation (from data): Nitrate reductase (EC 1.7.99.4)
Rationale: Specifically important for utilizing Sodium nitrate. Automated validation from mutant phenotype: the predicted function (1.7.99.4) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: nitrate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 930 PF04879: Molybdop_Fe4S4" amino acids 25 to 76 (52 residues), 55.2 bits, see alignment 1.1e-18 PF00384: Molybdopterin" amino acids 81 to 510 (430 residues), 257.1 bits, see alignment E=5e-80 PF01568: Molydop_binding" amino acids 624 to 735 (112 residues), 100.8 bits, see alignment E=9.4e-33 PF04324: Fer2_BFD" amino acids 873 to 923 (51 residues), 53.4 bits, see alignment 5.3e-18

Best Hits

KEGG orthology group: K00372, nitrate reductase catalytic subunit [EC: 1.7.99.4] (inferred from 82% identity to reh:H16_B0776)

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YP90 at UniProt or InterPro

Protein Sequence (930 amino acids)

>RR42_RS31800 Nitrate reductase (EC 1.7.99.4) (Cupriavidus basilensis FW507-4G11)
MNLSDIPVILSTGSPTLLSADASVTVTDTTCPYCGVGCGVRATRHADGSVEVAGDTSHGS
NQGRLCVKGSALAETVGLEGRLLHPQVRGADGALQPVSWDHALDKVAQGFKDIIAEHGPD
AVALYVSGQLLTEDYYVANKLMKGFIGSANIDTNSRLCMSSAVAGHKRAFGEDLVPGNYE
DLELADLVVLVGSNTAWCHPILFQRLLKAKEARPEMKLVVIDPRRTATCELADLHLPVKP
GTDVWLFNGLLHYLAQHGAVDQSFVDAHTSGLDAALAAAGPDCADIAKVAKACKLDPEAL
RGFYALFAATPKVVTGFSQGVNQSSAGTDKVNSIINCHLATGRVGQPGMGPFSLTGQPNA
MGGREVGGLANMLAAHMELADPLHREIVQSFWGSPAIADKPGLKAVELFEAIEQGRVKAV
WVIATNPVVSLPDADQARRALAKCQLVVSSDIMLRTDTNDAAHVLLPALGWGEKDGTVTS
SERRISRQRAFLPAPGEARADWRILCDVATRMGFDGFRFAGPHEIFDEHARLSTYRNAGS
SDQPHAPRMFDLAGLAGMSQKQFDALSPVQWPVMATPPGRLANASGGTARLFADGRFAHP
DGRARFIATAPRAPFHATDDEFPLVLNTGRVRDQWHTMTRTGKSPKLADHIAEPFVDMHP
QDALLSGVSEGKLARVTTRWGAMVARVQHSGGIPRGSVFVPIHWNGQFSSDARVGALVNP
VVDPVSGEPEFKHTPVRVEEFRVSWHGFVLSREDLQADELTYWTRVQGRQFQRYEFAGRE
NIADRAAWARQLLGIDDPNADWIEYEDRTAGIYHAGHVVDDRLQACLYVCTRPDLPSRAW
LSTLFAKDRLEDADRIGLLLGQPMEKGADAGPTVCSCFGVGRNTICDAVRKHDLKTPAEV
TACVKAGGNCGSCVPELKKLLAEIRVAEVA