Protein Info for RR42_RS31310 in Cupriavidus basilensis FW507-4G11

Annotation: formate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 945 PF13510: Fer2_4" amino acids 18 to 94 (77 residues), 55.3 bits, see alignment E=2.6e-18 PF00111: Fer2" amino acids 27 to 77 (51 residues), 25.6 bits, see alignment 4.4e-09 PF13187: Fer4_9" amino acids 158 to 216 (59 residues), 29.8 bits, see alignment 2.2e-10 PF12838: Fer4_7" amino acids 158 to 216 (59 residues), 31.5 bits, see alignment 8.8e-11 PF00037: Fer4" amino acids 200 to 217 (18 residues), 24.4 bits, see alignment (E = 8.9e-09) PF04879: Molybdop_Fe4S4" amino acids 232 to 281 (50 residues), 59 bits, see alignment 1.7e-19 TIGR01591: formate dehydrogenase, alpha subunit" amino acids 235 to 918 (684 residues), 762.5 bits, see alignment E=1.6e-233 PF00384: Molybdopterin" amino acids 285 to 723 (439 residues), 253.5 bits, see alignment E=1.4e-78 PF01568: Molydop_binding" amino acids 810 to 915 (106 residues), 92.3 bits, see alignment E=9.2e-30

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 87% identity to cti:RALTA_B1495)

MetaCyc: 65% identical to NAD-dependent formate dehydrogenase alpha subunit (Methylorubrum extorquens AM1)
1.2.1.2-RXN [EC: 1.17.1.9]

Predicted SEED Role

"tungsten-containing formate dehydrogenase alpha subunit" in subsystem Formate hydrogenase

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.1.9 or 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YL26 at UniProt or InterPro

Protein Sequence (945 amino acids)

>RR42_RS31310 formate dehydrogenase (Cupriavidus basilensis FW507-4G11)
MNALTRAERAQVDASSGTTVTFRLNGRDVCAGPGESLLKVAQREGFDIPHLCYKDGLDTA
GNCRACMVEIKGERVLAPSCCRYPAQGMEVLTASERALHSQRMVLALLQSDMREAHYTRN
NALDHWSARLDVGKPRFAPRAAVPADLSHPAIAVNLDACIQCTRCVRACREEQVNDVIGL
AFRGDQAAIVFDMGDPMGASTCVACGECVQACPTGALMPARDAALPVPDRQVESVCPYCG
VGCQLTYNVKDNRILFVEGRPGPANQMRLCVKGRYGFDYAQHPHRLTVPLVRREGAPKRG
DFVMDPARVMDVFREASWDEALALAGTSLMAIRDTYGKRALAGFGSAKGSNEEAYLFQKL
VRTGFGSNNVDHCTRLCHASSVAALLEGIGSGAVSNPVMDVARAQVVIVIGANPTVNHPV
AATWIKNAVKNGTRLIVADPRRGDLARFAHRFLQFEPDTDVALLNAMMHVIVAEGLVDQD
FIASRTIGYEELQRNVAAYSPELMAPVCGIDADTIRYVARLYASSKASMILWGMGISQHI
HGTDNARCLIALALMTGQIGRPGTGLHPLRGQNNVQGASDAGLIPMMYPDYHRVDDPEAV
ARFESLWGMTLDRQPGLTVVEVMRAIERGEVRGMYIMGENPAMSDPDAQHAREALAALDH
LVVQDIFLTETAYLADVVLPASAFPEKTGTFTNTDRTVQLGRQAIDPPGQARQDLWIIQQ
MAARLGLDWSYASVADVFDEMRHAMPSIGGITWERLQREGAVTYPCRAEGDPGEPVIFTD
AFPTASGRARFVPADIIPAAERPDQEYPVVLITGRQLEHWHTGSMTRRAGVLDAIEPEPV
ALVHPLDLAAIGGQPGDVVTLASRRGQVSLFARVDDGTPRGAVFVPFCYYEAAINKLTNA
ALDPFGKIPEFKYCAIRITLGGEVPRQSSFGGGQILAAGSKQGDG