Protein Info for RR42_RS29420 in Cupriavidus basilensis FW507-4G11

Annotation: 3-methylcrotonyl-CoA carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 666 PF00289: Biotin_carb_N" amino acids 7 to 115 (109 residues), 150.2 bits, see alignment E=8.8e-48 PF02786: CPSase_L_D2" amino acids 121 to 327 (207 residues), 252.9 bits, see alignment E=6.9e-79 PF07478: Dala_Dala_lig_C" amino acids 147 to 296 (150 residues), 35.9 bits, see alignment E=1.7e-12 PF02785: Biotin_carb_C" amino acids 341 to 445 (105 residues), 106.1 bits, see alignment E=3.3e-34 PF00364: Biotin_lipoyl" amino acids 588 to 652 (65 residues), 51.4 bits, see alignment E=2.3e-17

Best Hits

KEGG orthology group: K13777, geranyl-CoA carboxylase alpha subunit [EC: 6.4.1.5] (inferred from 66% identity to aaa:Acav_0072)

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4, 6.4.1.5

Use Curated BLAST to search for 6.4.1.4 or 6.4.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YCP4 at UniProt or InterPro

Protein Sequence (666 amino acids)

>RR42_RS29420 3-methylcrotonyl-CoA carboxylase (Cupriavidus basilensis FW507-4G11)
MKQPTPFQKILIANRGEIALRVMRSATALGYRTVAVYSSADAGARHVEEAGQAVYIGQAQ
PAQSYLRIDAIIEAARRSGADAVHPGYGFLAENAAFARACREAGLVFIGPSAESIVAMGN
KAGAKRLMMAADVPCIPGYQGEDQDEARLCAEAERIGFPVMIKATAGGGGRGMRLVPHAR
AFPELLRSARSEAQGAFGDPEVILERAVVEPRHIEIQILADRYGNAIHLGERDCSVQRRH
QKLIEEAPSPAVSAELRARMGATAVAAVKAIGYEGAGTLEFLLDRDGNYYFMEMNTRLQV
EHPVTEAITGLDLVALQLRIAAGEPLPLRQEDVRFSGHAIEVRLCAEDADQGFMPQSGEI
ALWQAPPALRVEHALGSGAAIPPYYDSMIAKLISYGGTRDEARRKLSQGLEDLVALGVTT
NQVFLGRCLAHPAFAAGEATTAFIGQHQDALLQPDAALRQRAAALAALLLYETSPERQPG
APAASLAPTLPIGLRYRVNGAEHQASLAYGAGNRFAVAMGESRFAFETIALGAHSVRFSC
DGLVESATFHRDASTLLLHYQGTPLRIEDHTRAAAARAGEAAGDGKLRASMNGRVVNVMV
AAGDQVEAGQPMVTLEAMKMEHIHVAPAAGRVGAVHVQVGDQAAAMRVIAEIEFEAPPAE
PVKAAA