Protein Info for RR42_RS28890 in Cupriavidus basilensis FW507-4G11

Annotation: acetone carboxylase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 714 PF05378: Hydant_A_N" amino acids 10 to 198 (189 residues), 131.8 bits, see alignment E=3.5e-42 PF01968: Hydantoinase_A" amino acids 223 to 509 (287 residues), 287.4 bits, see alignment E=1.8e-89 PF19278: Hydant_A_C" amino acids 522 to 713 (192 residues), 300 bits, see alignment E=9.2e-94

Best Hits

Swiss-Prot: 70% identical to ACXA_XANP2: Acetone carboxylase beta subunit (acxA) from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)

KEGG orthology group: K01473, N-methylhydantoinase A [EC: 3.5.2.14] (inferred from 90% identity to bph:Bphy_6150)

MetaCyc: 70% identical to acetone carboxylase beta subunit (Xanthobacter autotrophicus Py2)
Acetone carboxylase. [EC: 6.4.1.6]

Predicted SEED Role

"Acetone carboxylase, beta subunit (EC 6.4.1.6) / N-methylhydantoinase A (EC 3.5.2.14)" in subsystem Hydantoin metabolism (EC 3.5.2.14, EC 6.4.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.2.14, 6.4.1.6

Use Curated BLAST to search for 3.5.2.14 or 6.4.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YMJ9 at UniProt or InterPro

Protein Sequence (714 amino acids)

>RR42_RS28890 acetone carboxylase subunit beta (Cupriavidus basilensis FW507-4G11)
MEVQSQVQVMGIDAGGTMTDTFFVRGDGRFVVGKAQSNPEDESLAIFNSSEDALAHWQRG
VDDVYPELLTCVYSGTAMLNRVVQRKGLDVGLMVNKGFEHVHSMGRAIQSYLGYALEERI
HLNTHRYDEPLVPLSRTRGVTERTDVQGEIVIPLRENEVRQATRELVAAGSKAVVICFLQ
SHKNGTSEQQARDITRDELKKLGAEIPVFASVDYYPQRKESHRMNTTVLEAYAAEPSRQT
LKKVSDRFRKHGARFDLRVMATHGGTISWKAKELARTIVSGPIGGVIGSKLLGETLGYDN
IACSDIGGTSFDMALITKGNFAIAADPDMARLVLSLPLVTMDSVGAGAGSFVRLDPYSGA
IKLGPDSAGYRVGTCWPESGLDTVSVSDCHVVLGYLNPDNFLGGAIRLDVERARQHIKAQ
IADPLGLSVEDAAAGVIELLDLQLREYLRSNVSAKGYNPTEFVCFSYGGAGPVHTYGYTE
GLGFKDVIVPAWAAGFSAFGCACADFEYRYDKSVDLALPQDAAQEDKAGAAQTIQRAWSD
LAAKVIEEFKINGFKAEDVILRPGYRMQYMGQLNDLEISSPIVNAAVAGDWDQIVDAFEE
TYGRVYASSARSPELGFSVTGAIMRGMVITQKPVLPEDPEGGPKPPAQARLGTRPLYRQK
RWHDAVLWKMEALLPGNTITGPAIIESDATTFVVPEGFATTLDKHHLFHLKEVK