Protein Info for RR42_RS28845 in Cupriavidus basilensis FW507-4G11

Annotation: D-aminoacylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 497 PF07969: Amidohydro_3" amino acids 59 to 279 (221 residues), 72 bits, see alignment E=7.4e-24 amino acids 338 to 466 (129 residues), 65.6 bits, see alignment E=6.6e-22

Best Hits

Swiss-Prot: 51% identical to NDAD_ALCXX: D-aminoacylase (dan) from Alcaligenes xylosoxydans xylosoxydans

KEGG orthology group: K01436, amidohydrolase [EC: 3.5.1.-] (inferred from 86% identity to reh:H16_B1209)

Predicted SEED Role

"D-aminoacylase (EC 3.5.1.81)" (EC 3.5.1.81)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.-, 3.5.1.81

Use Curated BLAST to search for 3.5.1.- or 3.5.1.81

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YL33 at UniProt or InterPro

Protein Sequence (497 amino acids)

>RR42_RS28845 D-aminoacylase (Cupriavidus basilensis FW507-4G11)
MPELFDTLIRGVTLLDGSGAEARAADVALRAGRIARIAMLEDGADAIDPGSAAHVVQGAG
LVLAPGFIDVHTHDDTNVVRQPEMTPKLSQGVTTVIVGNCGISAAPVTLRGEPPDPMNLL
GTSDAFCYPDFAAYAAAVQAARPAVNVAALVGHTALRSNHMDRLDRTATPAEIAAMRAQL
QEALDHGAIGLSTGLAYANAFAATTDEVLALAEPLAHAGALYATHLRSEFADILEAMDEA
FRIGRHARVPVVISHMKCAGLENWGRSGEVLDALERAQRWQPVGCDCYPYTASSSTLDLK
QVTSDFDIMVTWSEPHPDMGGRLLADIAAEWDLGLEDAARRLQPAGAVYHCMEDADVDRI
LSHPATVVGSDGLPNDPLPHPRLWGAFPRVLGHYARDRELFPLTVAINKMTGLSAERFGL
AGRGLVREGYWADLVLFDAATIRDAASFTDPMQPAEGIACVWVNGELSWQQRKPTGARAG
RFLARTKPAGASAAPAQ