Protein Info for RR42_RS28545 in Cupriavidus basilensis FW507-4G11

Updated annotation (from data): 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4)
Rationale: Specifically important for utilizing L-Valine. Automated validation from mutant phenotype: the predicted function (3.1.2.4) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: enoyl-CoA hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K01692, enoyl-CoA hydratase [EC: 4.2.1.17] (inferred from 68% identity to cti:RALTA_B1106)

Predicted SEED Role

"3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 3.1.2.4)

MetaCyc Pathways

oleate β-oxidation (29/35 steps found)
superpathway of coenzyme A biosynthesis II (plants) (9/10 steps found)
adipate biosynthesis (5/5 steps found)
adipate degradation (5/5 steps found)
superpathway of phenylethylamine degradation (9/11 steps found)
benzoyl-CoA degradation I (aerobic) (6/7 steps found)
superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
benzoyl-CoA biosynthesis (3/3 steps found)
β-alanine biosynthesis II (5/6 steps found)
phenylacetate degradation I (aerobic) (7/9 steps found)
L-valine degradation I (6/8 steps found)
fatty acid β-oxidation I (generic) (5/7 steps found)
2-methyl-branched fatty acid β-oxidation (10/14 steps found)
valproate β-oxidation (6/9 steps found)
acrylate degradation I (3/5 steps found)
fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
fatty acid β-oxidation IV (unsaturated, even number) (3/5 steps found)
propanoyl-CoA degradation II (3/5 steps found)
3-phenylpropanoate degradation (6/10 steps found)
methyl ketone biosynthesis (engineered) (3/6 steps found)
(8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
benzoate biosynthesis I (CoA-dependent, β-oxidative) (4/9 steps found)
benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
platensimycin biosynthesis (7/26 steps found)
Spodoptera littoralis pheromone biosynthesis (4/22 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.17

Use Curated BLAST to search for 3.1.2.4 or 4.2.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YCA7 at UniProt or InterPro

Protein Sequence (384 amino acids)

>RR42_RS28545 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) (Cupriavidus basilensis FW507-4G11)
MRLNPNDAAQERPAAAPSADDEVRFDEINGIGLITLNRPRQLNALSYPMIGLLDAQLAAW
AARDDIAAVVLRGAGPKAFCAGGDIRALYDSFHAGTALHRQFFVDEYQLDYRLHCYPKPV
VALMDGIVMGGGMGLAQAAHLRVLTERSRVAMPETGIGLVPDVGASHFLSKLPLALALYV
GLTGVTLGAADTLLCKLADIAVPAASLEHFEQTLAAINRTGDVLADLRAALQATPDAGEQ
AAPLQSVLPAVLRHFRADASVAGLLDSLAAESDPAYADWAARTLDILRGRSPLMMAVTRE
LLLRGRDLDLADCFRMELGVVSHAFSQGDFIEGVRALIVDKDNAPRWRVKDASEVSEAVV
QSFFDSPWPREPHPLAMLGRSGQA