Protein Info for RR42_RS28225 in Cupriavidus basilensis FW507-4G11

Annotation: diguanylate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 725 TIGR00229: PAS domain S-box protein" amino acids 22 to 135 (114 residues), 59 bits, see alignment E=5.1e-20 PF08448: PAS_4" amino acids 25 to 130 (106 residues), 37.2 bits, see alignment E=1.1e-12 PF00989: PAS" amino acids 25 to 125 (101 residues), 41.1 bits, see alignment E=5.7e-14 PF13426: PAS_9" amino acids 29 to 128 (100 residues), 50.5 bits, see alignment E=7.8e-17 PF08447: PAS_3" amino acids 37 to 121 (85 residues), 27.9 bits, see alignment E=8.3e-10 PF12860: PAS_7" amino acids 228 to 286 (59 residues), 33.8 bits, see alignment 1.2e-11 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 290 to 445 (156 residues), 121.5 bits, see alignment E=2.8e-39 PF00990: GGDEF" amino acids 293 to 444 (152 residues), 136.5 bits, see alignment E=2.6e-43 PF00563: EAL" amino acids 471 to 700 (230 residues), 244.1 bits, see alignment E=4.6e-76

Best Hits

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YJE8 at UniProt or InterPro

Protein Sequence (725 amino acids)

>RR42_RS28225 diguanylate cyclase (Cupriavidus basilensis FW507-4G11)
MPHIQGPPGETELFRTLVLGVTDYAIYALDPAGTVSSWNPGAQRAKGYTRDEIVGAHFSC
FYTPEDRAAGLPEQALRQAREAGRFAAEGCWRLRKDGSRFWAHVLITPIFDRKGKQLGYA
KVTRDMTQQKADAERIAEVTRTLDLALENMSQGLCLFDPHGTIILINRRMDEIFRGAGAA
LVPGADFLTLCRAMAGAREREIAAPGVLAIRLGGNAQAEAGQPEDRAEQYAQALCERHRE
ILRRHQQWRSVEALPDGRAIALSHSVMADGSWVTTCEDVTEQRRIEAKIQYMARHDGLTG
LPNRSYFNDYLDEELQRAQKQGTRVATLGIDLDCFKEINDLHGHAAGDTVLRAVSQRIAS
QLEPGEFIARIGGDEFIAVKRFERQAELSDFSARLVTCVSEPVRLDGFDLTTGASIGIAI
YPGDGTHRDQLVSNTDLAMYRAKGSLSESICFYESRMDEAERERRALAGELWTAIAANQF
VLDYQVQTAIASGEAIGCEVLLRWHHPERGLIAPDSFIPLAEECGAILPIGEWVLRTACQ
QAAQWTFPRKISVNLSPVQITHGNLVTLVAQVLEETGLAAGRLELEITESTIIGDKERAL
DILRRIKALGVTIAIDDFGTGYSSLDTLRSFPFDKIKLDRTFIAEISDSQQAKAIIRAIL
ALGQSLAIPVLAEGVETDEQLSLLQAEGCDEAQGFLLGRPQPLEHFRRAHAVVGPRLVTA
QTPER