Protein Info for RR42_RS27845 in Cupriavidus basilensis FW507-4G11

Annotation: 5-carboxymethyl-2-hydroxymuconate isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 129 PF02962: CHMI" amino acids 2 to 126 (125 residues), 180.5 bits, see alignment E=7.7e-58

Best Hits

Swiss-Prot: 51% identical to HPCD_ECOLX: 5-carboxymethyl-2-hydroxymuconate Delta-isomerase (hpcD) from Escherichia coli

KEGG orthology group: K01826, 5-carboxymethyl-2-hydroxymuconate isomerase [EC: 5.3.3.10] (inferred from 77% identity to bmj:BMULJ_04136)

MetaCyc: 52% identical to 5-carboxymethyl-2-hydroxymuconate isomerase (Escherichia coli B)
5-carboxymethyl-2-hydroxymuconate Delta-isomerase. [EC: 5.3.3.10]

Predicted SEED Role

"5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10)" in subsystem 4-Hydroxyphenylacetic acid catabolic pathway or Aromatic amino acid degradation or Central meta-cleavage pathway of aromatic compound degradation (EC 5.3.3.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 5.3.3.10

Use Curated BLAST to search for 5.3.3.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YBU9 at UniProt or InterPro

Protein Sequence (129 amino acids)

>RR42_RS27845 5-carboxymethyl-2-hydroxymuconate isomerase (Cupriavidus basilensis FW507-4G11)
MPHIIVEYTDNIRADARVPALLKTINDVLIAQDGVFPIGGIRSRALALTDYRMADGEEDY
AFVHITLKIGAGRPEAARKAACDALFEAVKAHFAELYARRYLALSMELVEFNEGGSYKHN
NVHARFKKA