Protein Info for RR42_RS27150 in Cupriavidus basilensis FW507-4G11

Annotation: hemolysin D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 370 transmembrane" amino acids 28 to 49 (22 residues), see Phobius details PF16576: HlyD_D23" amino acids 67 to 310 (244 residues), 57.8 bits, see alignment E=1.5e-19 PF13533: Biotin_lipoyl_2" amino acids 67 to 115 (49 residues), 57.8 bits, see alignment 1.1e-19 PF13437: HlyD_3" amino acids 234 to 343 (110 residues), 48.4 bits, see alignment E=2.1e-16

Best Hits

Swiss-Prot: 35% identical to EMRA_ACIBT: Colistin resistance protein EmrA (emrA) from Acinetobacter baumannii (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 5377)

KEGG orthology group: K03543, multidrug resistance protein A (inferred from 75% identity to reh:H16_B1781)

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YLK3 at UniProt or InterPro

Protein Sequence (370 amino acids)

>RR42_RS27150 hemolysin D (Cupriavidus basilensis FW507-4G11)
MTSDITAERAGPAPIPVASKPRISRKRLILTGALIAAGLGAATYGQHWWTHGRFLQSTDD
AYVGGEVTVIAPKVPGYLAQVLVTDNQQVHAGDLLARIDDRDYRAALAKAEGAVAAQQAL
LANLDATRSLQEAVIGQARAAVTAVNADTLRSRDDQARYQSLAAKSAVSVQSAQKADSDY
KQALANTQKAQASLLAAQRQVDVIATQKLQAQAALAQAVAERDIARLNLGYTELRAPIDG
TIGNRRARVGAYAAGGAQLLSVVPARGLWVDANFKEGQLAHLRPGMRATVEADVLPGRVF
HGRVLSLAPATGAQFSVLPPENATGNFTKIVQRVPVRIMLDDNEAALAMLRPGLSVLAQV
DSRADAQVQP