Protein Info for RR42_RS26895 in Cupriavidus basilensis FW507-4G11

Annotation: 3-methylcrotonyl-CoA carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 674 PF00289: Biotin_carb_N" amino acids 1 to 110 (110 residues), 152.2 bits, see alignment E=2.3e-48 PF02786: CPSase_L_D2" amino acids 116 to 322 (207 residues), 256.5 bits, see alignment E=6.3e-80 PF07478: Dala_Dala_lig_C" amino acids 142 to 291 (150 residues), 39.5 bits, see alignment E=1.5e-13 PF02222: ATP-grasp" amino acids 143 to 292 (150 residues), 36.7 bits, see alignment E=1.1e-12 PF02785: Biotin_carb_C" amino acids 336 to 454 (119 residues), 129.3 bits, see alignment E=2.3e-41 PF00364: Biotin_lipoyl" amino acids 604 to 669 (66 residues), 59.6 bits, see alignment E=7.6e-20

Best Hits

KEGG orthology group: K01968, 3-methylcrotonyl-CoA carboxylase alpha subunit [EC: 6.4.1.4] (inferred from 82% identity to reu:Reut_A1463)

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4

Use Curated BLAST to search for 6.4.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YII8 at UniProt or InterPro

Protein Sequence (674 amino acids)

>RR42_RS26895 3-methylcrotonyl-CoA carboxylase (Cupriavidus basilensis FW507-4G11)
MFNKILIANRGEIACRVAATCRRLGIRTVAVYSDADADARHVALCDEAVHIGGAPARDSY
LRGDHIIEMAKATGAQAIHPGYGFLSENDGFAEACAEAGLVFIGPPASSIRAMGSKSAAK
RLMEAAAVPLVPGYHGEDQEPALLRREADRIGYPVLLKASAGGGGKGMRVVEASEGFEAA
LASVQREASASFGDDRVLVEKYLTRPRHIEIQVFADTHGNCVYLFERDCSVQRRHQKVLE
EAPAPGMSEERRRAMGEAAVAAAQAVGYVGAGTVEFIANQDGSFYFMEMNTRLQVEHPVT
EMITGQDLVEWQLRVAAGEPLPLRQDQLQIHGHALEARVYAENPDKGFLPSTGTLRYLRT
PAAVTFALGQDGNGPAGVRIDSGVREGDTISPYYDPMIAKLIVWGRDREEALARMAQALA
EYHVVGLSTNIAFLQRLVTSQAFRTADLDTGLIERNQAALFPPPAKVGTEVIALAVAALV
DADTRARRVDPADAHSPWQNAGAWRLNADATSQFRLRTGEGETLASLRSDGGKQWLTVGE
QTLPLRVTSLAGSHALDLGERTVKGSTYIEGDALYVFSGKQQTRLEVIDPLAHAGSADAE
GGKLVAPMPGRIIAVMVKAGTVIGKGDPLIVMEAMKMEHTLTAPADGVVQEVLYGIGDQV
QDGAQLVTLEDVAA