Protein Info for RR42_RS25850 in Cupriavidus basilensis FW507-4G11

Annotation: 3-carboxy-cis,cis-muconate cycloisomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00206: Lyase_1
  • amino acids 26 to 306 (281 residues), 186 bits, E=1.2e-58
• PF10397: ADSL_C
  • amino acids 372 to 449 (78 residues), 73 bits, E=2.1e-24

Best Hits

Swiss-Prot: 47% identical to TAD1_USTMD: Trans-aconitate decarboxylase 1 (TAD1) from Ustilago maydis

KEGG orthology group: K01857, 3-carboxy-cis,cis-muconate cycloisomerase [EC: 5.5.1.2] (inferred from 90% identity to rsl:RPSI07_mp0965)

MetaCyc: 47% identical to trans-aconitate decarboxylase (Ustilago maydis)
RXN-19902 [EC: 4.1.1.113]

Predicted SEED Role

"3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 5.5.1.2)

MetaCyc Pathways

• aromatic compounds degradation via β-ketoadipate (9/9 steps found)
• protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (25/35 steps found)
• toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
• itaconate biosynthesis II (1/3 steps found)
• superpathway of aerobic toluene degradation (19/30 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation

Isozymes

Compare fitness of predicted isozymes for: 5.5.1.2

Use Curated BLAST to search for 4.1.1.113 or 5.5.1.2

Sequence Analysis Tools

See A0A0C4YAU0 at UniProt or InterPro

Protein Sequence (456 amino acids)

>RR42_RS25850 3-carboxy-cis,cis-muconate cycloisomerase (Cupriavidus basilensis FW507-4G11)
MSTFSVPMASTVVDSILFRDAFGTARMREIFSDRALIQRYIDAEIALAKAEARVGVIPAE
AAEVIARESRIERIDFDHMREETDIVGYPILPLVHQLVGMCGDAGRYVHWGATTQDIMDT
AVALQVRDALDSIDTDIRELRAILADLAVKHRDTPMAGRTHLQQALPVTFGYKVAIWLAM
FDRHQQRLAELRPRVAVVEFAGAAGTLASLGDKGFAVQQAMAGELGLGVPATTWHVARDG
FAEAVNLLALITGSLGKIALDIMIMASTEFAEVYEPFVKGRGASSTMPQKRNPISSELML
AASKAVRQHAGLMVDAMVQDFERATGPWHAEWIAIPESFIFSSGALHQAKFALGGLVVDT
ERMKHNLGITRGLIVAEAVMMGMAPFTGRQQAHDIVYDACRTVNEKGGTLAEALMALPEV
TQHFDRAAIDRLTDPANYLGLAPQMVDRAVALSSGV