Protein Info for RR42_RS25640 in Cupriavidus basilensis FW507-4G11

Annotation: 3-hydroxyisobutyrate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 298 PF03446: NAD_binding_2" amino acids 2 to 160 (159 residues), 174 bits, see alignment E=3.7e-55 PF03807: F420_oxidored" amino acids 3 to 68 (66 residues), 27.8 bits, see alignment E=4.8e-10 TIGR01692: 3-hydroxyisobutyrate dehydrogenase" amino acids 5 to 291 (287 residues), 423.2 bits, see alignment E=2.7e-131 PF14833: NAD_binding_11" amino acids 164 to 291 (128 residues), 120.7 bits, see alignment E=6.7e-39

Best Hits

Swiss-Prot: 53% identical to SERDH_PSEAE: NAD-dependent L-serine dehydrogenase (PA0743) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00020, 3-hydroxyisobutyrate dehydrogenase [EC: 1.1.1.31] (inferred from 85% identity to bcj:BCAM1150)

MetaCyc: 69% identical to 3-hydroxyisobutyrate dehydrogenase subunit (Pseudomonas putida)
3-hydroxyisobutyrate dehydrogenase. [EC: 1.1.1.31]

Predicted SEED Role

"3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.1.1.31)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.31

Use Curated BLAST to search for 1.1.1.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YKR0 at UniProt or InterPro

Protein Sequence (298 amino acids)

>RR42_RS25640 3-hydroxyisobutyrate dehydrogenase (Cupriavidus basilensis FW507-4G11)
MEIAFIGLGNMGAPMAANLLKAGHALTVFDLDANAVAAALLAGATSAGSPREAAARADLV
ITMLPAAAHVRQVYLGEDGVLAGARPGVPMVDCSTIDPATVRTVAEAAASQDSPFADAPV
SGGTGGAQAGTLTFMVGADQPLFERIRPVLLAMGRNVVPCGAPGTGQVAKICNNLLLGIS
MMGVSEAMALGAALGIDPAVLAGIINTSTGRCWSSDSYNPYPGVQPAAPAARGYVGGFAA
DLMLKDLGLATDAARQAHQPLWMGALAQQLYQSMSQQGMGRLDFSACLKLYEKQDPSA