Protein Info for RR42_RS25255 in Cupriavidus basilensis FW507-4G11

Annotation: 1,4-butanediol diacrylate esterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: None (inferred from 94% identity to reu:Reut_C6049)

MetaCyc: 46% identical to methyl acetate hydrolase (Gordonia sp. TY-5)
RXN-17572 [EC: 3.1.1.114]

Predicted SEED Role

"Penicillin-binding protein"

MetaCyc Pathways

propane degradation I (1/4 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.114

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YIN6 at UniProt or InterPro

Protein Sequence (387 amino acids)

>RR42_RS25255 1,4-butanediol diacrylate esterase (Cupriavidus basilensis FW507-4G11)
MKANADPVLIRAVASGDTAGVAAAVITRDETLYTAAQGARTLGASAAMDLDTVMRIASMT
KPITSAAAMQLVEQGKLSLDGPAAAVIPELGALKVLEGWDTNGAPQLRDAKGQLTLRHLL
THTSGFTYEIWNADLERFYRTQNVPDVLTGQKAALRVPLCFDPGTRWEYGIGIDWVGQLV
EAASGMRLSDYFQRNITGPLGMDSTAFKISTAMRTRLAGMHSRGDDGELSVIDLGTVQDP
EFESGGAGLYSTANDYLKFMRMILNAGRGNDNQVLKPETVALMSRNAMGALRVSKLPTQL
PFFSRDMEFFPGVPKTWGLGFMINESATPTGRSAGSLAWAGLANTYFWIDPTLNIAGLAM
MQSLPSVDPRTVDVFYGFEKSVYASLI