Protein Info for RR42_RS24415 in Cupriavidus basilensis FW507-4G11

Annotation: pyruvate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 894 TIGR00759: pyruvate dehydrogenase (acetyl-transferring), homodimeric type" amino acids 9 to 892 (884 residues), 1376.6 bits, see alignment E=0 TIGR03186: alpha-ketoglutarate dehydrogenase" amino acids 9 to 893 (885 residues), 1550.6 bits, see alignment E=0 PF00456: Transketolase_N" amino acids 224 to 305 (82 residues), 22.5 bits, see alignment E=5.2e-09 PF17831: PDH_E1_M" amino acids 483 to 705 (223 residues), 315.3 bits, see alignment E=2e-98

Best Hits

Swiss-Prot: 54% identical to ODP1_ECOLI: Pyruvate dehydrogenase E1 component (aceE) from Escherichia coli (strain K12)

KEGG orthology group: K00163, pyruvate dehydrogenase E1 component [EC: 1.2.4.1] (inferred from 67% identity to bam:Bamb_6501)

MetaCyc: 54% identical to pyruvate dehydrogenase E1 component (Escherichia coli K-12 substr. MG1655)
Pyruvate dehydrogenase (acetyl-transferring). [EC: 1.2.1.104, 1.2.4.1]; 1.2.1.104 [EC: 1.2.1.104, 1.2.4.1]

Predicted SEED Role

"Pyruvate dehydrogenase E1 component (EC 1.2.4.1)" in subsystem Methionine Degradation or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.4.1

Use Curated BLAST to search for 1.2.1.104 or 1.2.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YA25 at UniProt or InterPro

Protein Sequence (894 amino acids)

>RR42_RS24415 pyruvate dehydrogenase (Cupriavidus basilensis FW507-4G11)
MAHGEQSLVDSDPQETAEWRDALLSLVDAVGRDRAHFILDMLATLGQHPSIGWKPLRGTP
YVNTIPVDRQPHFPGDLAMEERLGSIVRWNALAMVVRANKAYGELGGHIASYASAADLFE
VGFNHFFRARTDSFGGDLVFYQAHSAPGVYARAFLEGRLEERDLAHYRQEITANKAGARG
LCSYPHPWLMPDFWQFPTGSMGIGPISSIYQARFMRYLQHRGLLNTEGRKVWGVFGDGEM
DEPESMSALTLAARERLDNLVWVVNCNLQRLDGPVRGNGRIMDELEELFAGAGWNVIKLA
WGSDWDGLFARDTIGALIQAFSNTVDGQFQTFAAKDGRYNREHFFGQNEALASLAQGLTD
EQIDRLKRGGHDLVKIYAAYDAATRHRGQPTVILAQTKKGFGMGDAGQGKMTTHQQKKLD
RDDLISFRNRFQLPLTDEQTEALHFFKPAEDSDEMRYLHARREALGGYVPSRSIKAPPLK
VPALADYARFAIEADGKEMSTTMGFVRMLSNLLRDKTLAPRLVPIVADEARTFGMANLFK
QIGIYSSVGQHYEPEDIGSILSYREALDGQILEEGISEAGAISSWVAAATSYSVHGLPML
PFYIYYSMFGFQRIGDLIWAAADQRARGFLLGATAGRTTLGGEGLQHQDGGSLVTASMVP
NCRAYDPAFAGEFAVILDHGMRSMLENQEDVFFYVTLMNENYPQPSLPEGAAEGIIRGLY
LYGASQLAEAGEKVRLVGSGAILREVIAAADLLSHDWNIEAEIWSATSFSELAREARAVE
RWNRLNPEEEPRQSYVGCALSGETPVIVATDYVRAWPQLIAPYVDARLTVLGTDGFGRSD
TRSALRSFFEVDRHHIVLAALESLARSGRINPTLCKEAIERYGIAVDPKAPWEC