Protein Info for RR42_RS24325 in Cupriavidus basilensis FW507-4G11

Annotation: citryl-CoA lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03328: HpcH_HpaI
  • amino acids 18 to 241 (224 residues), 121.3 bits, E=3.7e-39
• PF15617: C-C_Bond_Lyase
  • amino acids 18 to 306 (289 residues), 39.6 bits, E=3.6e-14

Best Hits

KEGG orthology group: K01644, citrate lyase subunit beta / citryl-CoA lyase [EC: 4.1.3.34 4.1.3.6] (inferred from 60% identity to adn:Alide_0523)

MetaCyc: 44% identical to malyl coenzyme A lyase subunit (Methylorubrum extorquens AM1)
Malyl-CoA lyase. [EC: 4.1.3.24]

Predicted SEED Role

"Malyl-CoA lyase (EC 4.1.3.24)" in subsystem Photorespiration (oxidative C2 cycle) or Serine-glyoxylate cycle (EC 4.1.3.24)

MetaCyc Pathways

• formaldehyde assimilation I (serine pathway) (10/13 steps found)
• citrate degradation (2/2 steps found)
• methylaspartate cycle (14/19 steps found)
• 3-hydroxypropanoate cycle (8/13 steps found)
• glyoxylate assimilation (7/13 steps found)
• reductive TCA cycle II (6/12 steps found)
• superpathway of the 3-hydroxypropanoate cycle (10/18 steps found)
• ethylmalonyl-CoA pathway (4/11 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (3/14 steps found)

KEGG Metabolic Maps

• C5-Branched dibasic acid metabolism
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.34, 4.1.3.6

Use Curated BLAST to search for 4.1.3.24 or 4.1.3.34 or 4.1.3.6

Sequence Analysis Tools

See A0A0C4YI63 at UniProt or InterPro

Protein Sequence (307 amino acids)

>RR42_RS24325 citryl-CoA lyase (Cupriavidus basilensis FW507-4G11)
MHQTSPRPTRRRRVQLATPGSSEKMMEKAASSNADHVFLDLEDAVAPNQKVAARKKIIDA
LNHLDWQGKVRCVRINDLSTEYAYEDIIEIVEGAGHNLDTIMMTKVNGPADVLFADRLLS
MMEKKLRLPRRIGLEALIEEVPGLQNVEAIAQSTERLECLIFGMGDFSAAMGVNLESIGN
TGGYPGDIWHYARFRLIMACRAADIDPVDGPWAEFRDSEGYRNECRRAGTLGCVGKWAIH
PSQIEIALAEFSPDPAKVAQARKLESAYVQAEAEGLGAINVDGVMVDVATMRLLRNTVLK
KAQMYGL