Protein Info for RR42_RS23725 in Cupriavidus basilensis FW507-4G11

Annotation: phenylacetate-CoA oxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 334 TIGR02156: phenylacetate-CoA oxygenase, PaaG subunit" amino acids 29 to 317 (289 residues), 530.8 bits, see alignment E=3.5e-164 PF05138: PaaA_PaaC" amino acids 39 to 313 (275 residues), 325.9 bits, see alignment E=8.5e-102

Best Hits

Swiss-Prot: 64% identical to PAAA_ECOLI: 1,2-phenylacetyl-CoA epoxidase, subunit A (paaA) from Escherichia coli (strain K12)

KEGG orthology group: K02609, phenylacetic acid degradation protein (inferred from 87% identity to bcj:BCAL0216)

MetaCyc: 68% identical to ring 1,2-phenylacetyl-CoA epoxidase PaaA subunit (Pseudomonas sp. Y2)
RXN0-2042 [EC: 1.14.13.149]

Predicted SEED Role

"Phenylacetate-CoA oxygenase, PaaG subunit"

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.14.13.149

Use Curated BLAST to search for 1.14.13.149

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YHX0 at UniProt or InterPro

Protein Sequence (334 amino acids)

>RR42_RS23725 phenylacetate-CoA oxygenase (Cupriavidus basilensis FW507-4G11)
MYTQSLDIPGNAQPLETVQADVSPEQAHFDAVMDADGKIEAQDWMPDAYRKTLIRQISQH
AHSEIIGMQPEGNWISRAPSLKRKAILLAKVQDEGGHGLYLYSAAETMGVSRDQLVDALH
AGKAKYSSIFNYPTPTWADVGVIGWLVDGAAIMNQIPLCRCTYGPYARAMIRICKEESFH
QRQGFDALLSMMKGTQAQKDMVQESVNRWWWPVLMMFGPSDKDSIHSGQSFKWGIKRISN
DDLRQKFVDATVEQAKILGVTLPDPDLKWNEARGAHDYGAIDWEEFWRVVNGDGPCNKER
LATRVKAHNDGAWVREAALAHAEKQAARARKQAA