Protein Info for RR42_RS23490 in Cupriavidus basilensis FW507-4G11

Annotation: NADH oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 339 transmembrane" amino acids 212 to 230 (19 residues), see Phobius details PF00111: Fer2" amino acids 10 to 87 (78 residues), 65 bits, see alignment E=7.2e-22 PF00970: FAD_binding_6" amino acids 111 to 203 (93 residues), 54.4 bits, see alignment E=2e-18 PF00175: NAD_binding_1" amino acids 213 to 315 (103 residues), 78.6 bits, see alignment E=8.6e-26

Best Hits

Swiss-Prot: 65% identical to BENC_ACIAD: Benzoate 1,2-dioxygenase electron transfer component (benC) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K05784, benzoate 1,2-dioxygenase electron transfer component (inferred from 79% identity to reh:H16_A1961)

MetaCyc: 53% identical to XylZ (Pseudomonas putida mt-2)
Benzoate 1,2-dioxygenase. [EC: 1.14.12.10]

Predicted SEED Role

"benzoate dioxygenase, ferredoxin reductase component" in subsystem Benzoate degradation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.12.10

Use Curated BLAST to search for 1.14.12.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YGV0 at UniProt or InterPro

Protein Sequence (339 amino acids)

>RR42_RS23490 NADH oxidase (Cupriavidus basilensis FW507-4G11)
MEYNIALQFEDGVTRFITCTANETLSDAAYRQQINIPLDCRDGACGTCRGLCESGEYDLP
ASSYIEDALTPEEAAKGYVLACQTRPRSDCVIKVPASSAACKTGVTRYQGKLAAVDKLSD
STIGFSIDLGEAAAPTFLAGQYVNVDIPGTGLTRSYSFSSAPGAARTSFVVRNVPNGRMS
EFLSNEALPGQPISFAGPYGSFYLREVARPVLFLAGGTGIAPFLSMLDVLAANGSPHPIR
LVYGVTHEIDLVALAQLDRAKDQLAGFEYRTCVLDPVSNETRKGYVTQHVERDWLNGGDV
DIYLCGPVAMVDAVRAWLQDAGVTPASFHYEKFSASNAA