Protein Info for RR42_RS22865 in Cupriavidus basilensis FW507-4G11

Annotation: 2-amino-3-ketobutyrate CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 TIGR01822: glycine C-acetyltransferase" amino acids 8 to 399 (392 residues), 647.1 bits, see alignment E=4.7e-199 PF00155: Aminotran_1_2" amino acids 43 to 389 (347 residues), 227.2 bits, see alignment E=1.9e-71

Best Hits

Swiss-Prot: 68% identical to KBL_SALTY: 2-amino-3-ketobutyrate coenzyme A ligase (kbl) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00639, glycine C-acetyltransferase [EC: 2.3.1.29] (inferred from 84% identity to bgf:BC1003_4758)

MetaCyc: 68% identical to 2-amino-3-ketobutyrate CoA ligase (Escherichia coli K-12 substr. MG1655)
Glycine C-acetyltransferase. [EC: 2.3.1.29]

Predicted SEED Role

"2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29)" in subsystem Glycine Biosynthesis or Glycine and Serine Utilization (EC 2.3.1.29)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.29

Use Curated BLAST to search for 2.3.1.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YGF4 at UniProt or InterPro

Protein Sequence (400 amino acids)

>RR42_RS22865 2-amino-3-ketobutyrate CoA ligase (Cupriavidus basilensis FW507-4G11)
MRDRYLSHLRDTLDTIRADGFHKSERVIASPQAADIRLAGGEHVLNFCANNYLGLADDHR
LIEAARRGLEQDGFGMASVRFICGTQSVHKTLEAVLAKFLHTGDSILYSSCFDANGGLFE
TLLDDQDAVISDELNHASIIDGVRLCKARRYRYRNNDMADLETRLQEADAAGARFKLIAT
DGVFSMDGIIADLKSICDLADRYGALVMVDDSHAVGFVGEHGRGTPELCGVEGRVDIITG
TLGKALGGASGGYVAAHREIVELLRQRSRPYLFSNSLAPSICAATLKVLELLASEEGAAL
RARVRENGEHFRREMTALGFTLVPGQHPIIPVMLGDAALASGMADALLAEHVYVVGFSFP
VVPKGRARIRTQMSAAHTAEQIERAVAAFAKVGRKFGAIA