Protein Info for RR42_RS22630 in Cupriavidus basilensis FW507-4G11

Annotation: 3-hydroxyacyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00378: ECH_1
  • amino acids 9 to 179 (171 residues), 118.7 bits, E=7.3e-38
• PF16113: ECH_2
  • amino acids 23 to 182 (160 residues), 87.9 bits, E=2.5e-28
• PF02737: 3HCDH_N
  • amino acids 296 to 472 (177 residues), 211.7 bits, E=2e-66
• PF00725: 3HCDH
  • amino acids 476 to 569 (94 residues), 83.8 bits, E=2.5e-27
  • amino acids 605 to 690 (86 residues), 28.3 bits, E=5.1e-10

Best Hits

KEGG orthology group: K07516, 3-hydroxyacyl-CoA dehydrogenase [EC: 1.1.1.35] (inferred from 73% identity to axy:AXYL_04661)

Predicted SEED Role

"Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation or Butanol Biosynthesis (EC 1.1.1.35, EC 4.2.1.17, EC 5.1.2.3, EC 5.3.3.8)

MetaCyc Pathways

• oleate β-oxidation (29/35 steps found)
• adipate biosynthesis (5/5 steps found)
• adipate degradation (5/5 steps found)
• superpathway of phenylethylamine degradation (9/11 steps found)
• benzoyl-CoA degradation I (aerobic) (6/7 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• fatty acid salvage (5/6 steps found)
• phenylacetate degradation I (aerobic) (7/9 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (4/5 steps found)
• fatty acid β-oxidation III (unsaturated, odd number) (1/1 steps found)
• L-valine degradation I (6/8 steps found)
• fatty acid β-oxidation I (generic) (5/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (10/14 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (7/10 steps found)
• L-isoleucine degradation I (4/6 steps found)
• propanoate fermentation to 2-methylbutanoate (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• oleate β-oxidation (thioesterase-dependent, yeast) (1/2 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• valproate β-oxidation (6/9 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (3/5 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• fatty acid β-oxidation IV (unsaturated, even number) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• L-tryptophan degradation III (eukaryotic) (10/15 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• pyruvate fermentation to butanoate (4/7 steps found)
• oleate β-oxidation (reductase-dependent, yeast) (1/3 steps found)
• 3-phenylpropanoate degradation (6/10 steps found)
• 6-gingerol analog biosynthesis (engineered) (3/6 steps found)
• methyl ketone biosynthesis (engineered) (3/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
• fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) (2/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (17/26 steps found)
• oleate β-oxidation (isomerase-dependent, yeast) (1/4 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• androstenedione degradation I (aerobic) (16/25 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (10/17 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (7/13 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (4/9 steps found)
• glycerol degradation to butanol (9/16 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
• pyruvate fermentation to butanol I (3/8 steps found)
• gallate degradation III (anaerobic) (5/11 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• superpathway of testosterone and androsterone degradation (17/28 steps found)
• 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (4/10 steps found)
• 2-methylpropene degradation (2/8 steps found)
• methyl tert-butyl ether degradation (3/10 steps found)
• L-glutamate degradation VII (to butanoate) (4/12 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (7/17 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (6/16 steps found)
• 10-cis-heptadecenoyl-CoA degradation (yeast) (2/12 steps found)
• 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (2/12 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• toluene degradation VI (anaerobic) (5/18 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• cholesterol degradation to androstenedione I (cholesterol oxidase) (4/17 steps found)
• androstenedione degradation II (anaerobic) (10/27 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (20/42 steps found)
• platensimycin biosynthesis (7/26 steps found)
• Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (4/22 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (20/47 steps found)
• superpathway of cholesterol degradation III (oxidase) (12/49 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Bile acid biosynthesis
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.35, 4.2.1.17, 5.1.2.3, 5.3.3.8

Use Curated BLAST to search for 1.1.1.35 or 4.2.1.17 or 5.1.2.3 or 5.3.3.8

Sequence Analysis Tools

See A0A0C4YG98 at UniProt or InterPro

Protein Sequence (696 amino acids)

>RR42_RS22630 3-hydroxyacyl-CoA dehydrogenase (Cupriavidus basilensis FW507-4G11)
MPTVQYGRQGRIAILLLDNPPVNGLGDTVRLGLFDGIAQATQDDAVAAIVILGAGKVFCG
GADIRQFNAPAASAQPTLRQVIARIEQSAKPVVAAIHGVALGGGMELALGCHYRIASADA
SLGLPEVNLGLVPGGGGTQRLPRLIGVPAALELIQGGKPVRGDKAVALGMADALIDGDPL
AAAMAFAERVAGLPGAHPVIARRPCADAAGVDFAARSAAVSAKARNALAQRAAIACVQAA
TRLPIDAGLDDERARFDQLVASTESKALRHLFFAEREAPKVAALPAGASLRTVARVGILG
AGTMGAGIAMAFANAGIAVTLLEQQQAALDRGLAMIRRNYEITAAKGKLTAQEIAQRMAC
ITPTLADAALADADLVIEAVFEDMAVKKAVFARLDALCKPGAILATNTSRLSIDVIAAST
SRPQDVIGLHFFSPANVMKLLEVVQGERTAPEVIATCMKMARAIGKIPVLVRVCEGFVGN
RMLTPYWREAGFLLEEGASPQQVDGALTRFGMAMGPLAMADLAGMDINWATRKRLAPTRP
AHLRYSKVADRICEQGRFGQKTNAGYYRYEAGSRAPLPDPAVDALIRACAAEAGIERREV
SDEEIVERCMLALANEGARILEEGIARRAADIDVVYVHGYGFPAWRGGPMFHAETIGLGR
TLEKIRALNAIHGEHWTPAPLLVRLVAQGRETFAAG