Protein Info for RR42_RS21295 in Cupriavidus basilensis FW507-4G11

Updated annotation (from data): L-lactate dehydrogenase, LutA subunit
Rationale: Specifically important for utilization of L-lactate or D,L-lactate. (Also important on various nitrogen sources with lactate as the carbon source.) This is related to the LutABC system from Bacillus subtilis (PMC3347220, PMC2668416).
Original annotation: oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 262 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF02754: CCG" amino acids 14 to 94 (81 residues), 49.3 bits, see alignment E=2.2e-17 amino acids 146 to 230 (85 residues), 46.8 bits, see alignment E=1.4e-16

Best Hits

Swiss-Prot: 42% identical to LUTA_BACC1: Lactate utilization protein A (lutA) from Bacillus cereus (strain ATCC 10987 / NRS 248)

KEGG orthology group: None (inferred from 84% identity to reh:H16_B0093)

Predicted SEED Role

"Predicted L-lactate dehydrogenase, Fe-S oxidoreductase subunit YkgE" in subsystem Lactate utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YIN5 at UniProt or InterPro

Protein Sequence (262 amino acids)

>RR42_RS21295 L-lactate dehydrogenase, LutA subunit (Cupriavidus basilensis FW507-4G11)
MKQRRYPPAPAQVYLFATCLVDMFVPQAGLDAVRLLEREGLTVHFPRGQSCCGQPAYSSG
NPEQARAVALAQLDLFAEPWPVIVPSGSCAGMMRHHWPQLFAQDPVAGPKAALLAERVYE
LSEFLLHVLKVRFDVSGVAGQPPETVVLHTSCAARREMGTRDHGVALVDALPGVTRTEHQ
RESECCGFGGTFSLKHPDISGAMVQDKIASACATGCDRLVSADCGCLLNIGHAARHQGAP
LPVEHIASFLWRRTGGAAKEQA