Protein Info for RR42_RS21205 in Cupriavidus basilensis FW507-4G11

Annotation: 2-isopropylmalate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 565 TIGR00970: 2-isopropylmalate synthase" amino acids 5 to 549 (545 residues), 785.4 bits, see alignment E=1.6e-240 PF00682: HMGL-like" amino acids 32 to 311 (280 residues), 246 bits, see alignment E=6.7e-77 PF22615: IPMS_D2" amino acids 328 to 391 (64 residues), 118.3 bits, see alignment E=2.2e-38 PF08502: LeuA_dimer" amino acids 421 to 550 (130 residues), 93.7 bits, see alignment E=1.4e-30

Best Hits

Swiss-Prot: 84% identical to LEU1_CUPTR: 2-isopropylmalate synthase (leuA) from Cupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CIP 107171 / LMG 19424 / R1)

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 84% identity to reh:H16_B0081)

Predicted SEED Role

"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.3.13

Use Curated BLAST to search for 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YIL7 at UniProt or InterPro

Protein Sequence (565 amino acids)

>RR42_RS21205 2-isopropylmalate synthase (Cupriavidus basilensis FW507-4G11)
MLRNPATKYRPATAVDIADRTWPAKRITRAPVWMSTDLRDGNQALIEPMNPQRKLRFFEQ
LVKIGLKQIEVAFPSASQTDFDFVRMLIEEDRIPDDVTIVVLTQAREDLIRRTIESVRGA
ARAVVHLYNPIAPAFRRIVFGASREEVKEIALEGTRLIKALTDGMPETAWSYEYSPETFS
MTELDFSLEVCDAVSAIWQPGPTRPLILNLPTTVECSTPNIFADQVEWMHRHLARRADIV
LSVHPHNDRGTAVAAAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVDPGLD
FSDIDEVRQCVEDCNQLPVHPRHPYVGDLVFTAFSGSHQDAIRKGFAQHKADAIWEVPYL
PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQVHGIYMPRRLQIEFSRAVQSMTDESGLE
ASADDLYGLFKREYLEHEAPLRYMGHQMVSGSDGQVEIEVALLRDGEALSARGRGNGPID
AFVSGLNGALDMPVRVMDYHEHALSCGANAAAACYVEVRVGDSATGFGAGIDASIVTASL
KAVVSGVNRHLLAASPSQREDALVD