Protein Info for RR42_RS21015 in Cupriavidus basilensis FW507-4G11

Annotation: mannose-1-phosphate guanyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01479: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase
  • amino acids 7 to 473 (467 residues), 672.3 bits, E=1.8e-206
• PF00483: NTP_transferase
  • amino acids 9 to 291 (283 residues), 176.1 bits, E=2e-55
• PF12804: NTP_transf_3
  • amino acids 10 to 145 (136 residues), 33.3 bits, E=1.1e-11
• PF01050: MannoseP_isomer
  • amino acids 320 to 469 (150 residues), 234.3 bits, E=1.2e-73
• PF07883: Cupin_2
  • amino acids 386 to 453 (68 residues), 42.7 bits, E=7.5e-15

Best Hits

Swiss-Prot: 59% identical to XANB_XANCB: Xanthan biosynthesis protein XanB (xanB) from Xanthomonas campestris pv. campestris (strain B100)

KEGG orthology group: None (inferred from 57% identity to bgf:BC1003_0773)

MetaCyc: 52% identical to phosphomannose isomerase / GDP-D-mannose pyrophosphorylase (Pseudomonas aeruginosa)
Mannose-6-phosphate isomerase. [EC: 5.3.1.8]; Mannose-1-phosphate guanylyltransferase. [EC: 5.3.1.8, 2.7.7.13]

Predicted SEED Role

"Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)" in subsystem Alginate metabolism or Mannose Metabolism (EC 2.7.7.22)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (11/11 steps found)
• GDP-mannose biosynthesis (4/4 steps found)
• β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation (2/3 steps found)
• D-mannose degradation I (1/2 steps found)
• D-mannose degradation II (1/2 steps found)
• 1,5-anhydrofructose degradation (3/5 steps found)
• mannitol degradation II (2/4 steps found)
• superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (9/14 steps found)
• mannitol biosynthesis (1/3 steps found)
• β-(1,4)-mannan degradation (2/7 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.22, 5.3.1.8

Use Curated BLAST to search for 2.7.7.13 or 2.7.7.22 or 5.3.1.8

Sequence Analysis Tools

See A0A0C4Y894 at UniProt or InterPro

Protein Sequence (474 amino acids)

>RR42_RS21015 mannose-1-phosphate guanyltransferase (Cupriavidus basilensis FW507-4G11)
MADDKQIVPVILCGGAGSRLWPLSRDGYPKQFLRLLGQDSLLQDTLLRVREIPGAQAPIL
VSNEAHRFVVAEQAREAGCALCAIVLEPHPRNTAPAAAVAALQARSGGSDPVLLVLPSDH
LIRDRAAFARTVCQASAVAATGVVVTLGVVPTFAATGYGYIKAVPSNTADLLSVTRFVEK
PSLEMATGYLQEGNCFWNSGIFLFRASVYLAALERFAPDILLAAQEAMVRGHRDLDFFRL
DTDAFAACRSDSIDYAVMEHLRDAQMAPLSADWSDVGSWDAVWKAAEKTTDGNVAIGDVV
THHAQGCLVHASHRMVAVAGLDDVMVVETPDVVLVVHKDHCQDVKQLVEAVRAQGRPEAT
QHRKVYRPWGAYDAVDHGTRYQVKRITVKPGAKLSLQLHHHRAEHWVVVSGTARVRRGDV
EYLLTEDQSTYIPIGEAHSLENPGRIPLELIEIQSGSYLGEDDIIRMADIYGRT