Protein Info for RR42_RS20295 in Cupriavidus basilensis FW507-4G11

Annotation: alpha/beta hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF12146: Hydrolase_4
  • amino acids 56 to 287 (232 residues), 232.3 bits, E=7.5e-73
• PF12697: Abhydrolase_6
  • amino acids 61 to 263 (203 residues), 54 bits, E=5.6e-18
• PF00561: Abhydrolase_1
  • amino acids 61 to 172 (112 residues), 45.1 bits, E=1.6e-15

Best Hits

KEGG orthology group: None (inferred from 77% identity to cti:RALTA_A3121)

Predicted SEED Role

"Lysophospholipase (EC 3.1.1.5); Monoglyceride lipase (EC 3.1.1.23); putative" (EC 3.1.1.23, EC 3.1.1.5)

MetaCyc Pathways

• monoacylglycerol metabolism (yeast) (2/4 steps found)
• phospholipid remodeling (phosphatidylethanolamine, yeast) (2/4 steps found)
• triacylglycerol degradation (1/3 steps found)

KEGG Metabolic Maps

• Glycerolipid metabolism
• Glycerophospholipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.23, 3.1.1.5

Use Curated BLAST to search for 3.1.1.23 or 3.1.1.5

Sequence Analysis Tools

See A0A0C4YLH5 at UniProt or InterPro

Protein Sequence (311 amino acids)

>RR42_RS20295 alpha/beta hydrolase (Cupriavidus basilensis FW507-4G11)
MDRPSTAAPAEPTDVGGPAVAAPARTATPVESRQRMRDGTELFLRTWQPDPELFPEPAGS
LLLVHGLAEHCGRYQHVAELLCGLGLRVRAYDQRGHGASGGPRMVAEHPDVFVEDLTEIH
DAAVGQWRELPFLLGHSMGGLVAARFATARVRPIRALVLSSPALALRLSAPMLALHRMLL
TLVPRLRVPNPIDARYLSHDAAVVAQYRADPLVQGTISAGVLETFVRGMAQAQTDAPMLE
APMLMLVAGDDRIVDPAGSRRFFDNAPPDLRDALWFDTGYHEIFNEAQPLRGEVLAALTG
WLRKHLQPQAA