Protein Info for RR42_RS19870 in Cupriavidus basilensis FW507-4G11

Annotation: pyruvate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01064: pyruvate kinase
  • amino acids 5 to 470 (466 residues), 500.1 bits, E=2.8e-154
• PF00224: PK
  • amino acids 5 to 322 (318 residues), 367.3 bits, E=6.8e-114
• PF02887: PK_C
  • amino acids 355 to 467 (113 residues), 109.4 bits, E=1.2e-35

Best Hits

Swiss-Prot: 55% identical to KPYK_METEA: Pyruvate kinase (pyk) from Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)

KEGG orthology group: K00873, pyruvate kinase [EC: 2.7.1.40] (inferred from 91% identity to reu:Reut_A3295)

Predicted SEED Role

"Pyruvate kinase (EC 2.7.1.40)" in subsystem Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.1.40)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (15/17 steps found)
• Entner-Doudoroff pathway I (9/9 steps found)
• homolactic fermentation (11/12 steps found)
• superpathway of anaerobic sucrose degradation (16/19 steps found)
• Bifidobacterium shunt (13/15 steps found)
• glycolysis II (from fructose 6-phosphate) (10/11 steps found)
• glycolysis III (from glucose) (10/11 steps found)
• heterolactic fermentation (15/18 steps found)
• anaerobic energy metabolism (invertebrates, cytosol) (7/7 steps found)
• superpathway of glucose and xylose degradation (14/17 steps found)
• glycolysis I (from glucose 6-phosphate) (11/13 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (15/19 steps found)
• Rubisco shunt (8/10 steps found)
• mixed acid fermentation (12/16 steps found)
• glycolysis VI (from fructose) (8/11 steps found)
• glycolysis IV (7/10 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
• Entner-Doudoroff pathway III (semi-phosphorylative) (6/9 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (17/26 steps found)
• superpathway of N-acetylneuraminate degradation (14/22 steps found)
• superpathway of hexitol degradation (bacteria) (11/18 steps found)
• Entner-Doudoroff pathway II (non-phosphorylative) (4/9 steps found)
• glycerol degradation to butanol (9/16 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Carbon fixation in photosynthetic organisms
• Glycolysis / Gluconeogenesis
• Purine metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.40

Use Curated BLAST to search for 2.7.1.40

Sequence Analysis Tools

See A0A0C4YEW3 at UniProt or InterPro

Protein Sequence (492 amino acids)

>RR42_RS19870 pyruvate kinase (Cupriavidus basilensis FW507-4G11)
MRRQRKAKIVATLGPASTDIEVIRQLFEAGADVFRLNFSHGSHDDHKRRYDAVRQVEAET
GRPIAVLADLQGPKLRIGTFAAGKVAVKAGDAFVLDSDPSPGDATRVHLPHPELFRVAAP
GQSLLIDDGKVQLKIEAVASGSIATRVVNHGTLSDRKGVNVPDAVIPIPALTEKDRKDLD
FALALGADWIALSFVQRPADIVEAREIIGTRAGVLSKIEKPAALQQLEEIVRVSDAVMVA
RGDLGVELPPERVPGVQKRILRICRQHGKPIVIATQMLESMIDSPVPTRAEASDVASAIY
DGADAVMLSAESANGRHPVPAVAIMNRIITEVERDPLYRNLLDAGHEAPLSTRQDAICAA
LREVTQIIGAVATVTYTSSGSTALRAARERPCAPIVSITPNLEIARRLAIAWGVHSTVSP
DVQSVDEMVDAATRAALAEGYAEPGDQITIAAGMPFGQGGTTNLLRVAEIGGAQPGKTPG
QATASTAPAVTA