Protein Info for RR42_RS18085 in Cupriavidus basilensis FW507-4G11

Annotation: UDP-N-acetylmuramoylalanine--D-glutamate ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01087: UDP-N-acetylmuramoylalanine--D-glutamate ligase
  • amino acids 9 to 498 (490 residues), 373.1 bits, E=1.1e-115
• PF21799: MurD-like_N
  • amino acids 9 to 102 (94 residues), 50 bits, E=4.5e-17
• PF08245: Mur_ligase_M
  • amino acids 127 to 262 (136 residues), 84.5 bits, E=1.5e-27
• PF02875: Mur_ligase_C
  • amino acids 364 to 477 (114 residues), 58 bits, E=2.9e-19

Best Hits

Swiss-Prot: 86% identical to MURD_CUPNJ: UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) from Cupriavidus necator (strain JMP 134 / LMG 1197)

KEGG orthology group: K01925, UDP-N-acetylmuramoylalanine--D-glutamate ligase [EC: 6.3.2.9] (inferred from 86% identity to reu:Reut_A2981)

Predicted SEED Role

"UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.9)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• D-Glutamine and D-glutamate metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.9

Sequence Analysis Tools

See A0A0C4Y6K1 at UniProt or InterPro

Protein Sequence (508 amino acids)

>RR42_RS18085 UDP-N-acetylmuramoylalanine--D-glutamate ligase (Cupriavidus basilensis FW507-4G11)
MFGELQKPHVLVLGLGESGLAMARWCGLYGAPVRVADTREAPANLVFLQAELPSAEFHGG
ALAEALLDGIELVTISPGLSPLESDTAALLAAARERGIPVWGEIELFARALAHLRAESGY
APKLLAITGTNGKTTTTALTGRLVERAGKSVAVAGNISPSALDKLAACIASATLPEVWVL
ELSSFQLETTHTLAPHAATVLNITQDHLDWHGSMQAYAAAKARIFGPAPAPGEAGCLQIL
NRNDRLSLDMARPASALVTFGNDLPELPGCFGIQREGGMPWLVVAEPDSEAEGENKPRRR
KAAAAESVEAVPVRHKRLMPADALHIRGMHNATNAMAALALCRAIDLPMNALLHGLREYR
GEPHRVEWVATVDDVEFFDDSKGTNVGATVAALSGLDKRVVLIAGGEGKGQDFSPLAAPV
AQYARAVVLIGKDAGELRRALQDTGVTLADAATLEQAVTLAASAAQGGDAVLLSPACASL
DMFRNYVHRAEVFCAAVEELAMSRGIVP