Protein Info for RR42_RS17545 in Cupriavidus basilensis FW507-4G11

Annotation: leucyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 878 TIGR00396: leucine--tRNA ligase" amino acids 8 to 878 (871 residues), 1059.1 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 15 to 217 (203 residues), 84.5 bits, see alignment E=1.7e-27 amino acids 437 to 592 (156 residues), 32.6 bits, see alignment E=1e-11 amino acids 639 to 679 (41 residues), 28.5 bits, see alignment 1.7e-10 PF09334: tRNA-synt_1g" amino acids 44 to 177 (134 residues), 67.2 bits, see alignment E=3e-22 PF13603: tRNA-synt_1_2" amino acids 226 to 422 (197 residues), 217.7 bits, see alignment E=2.5e-68 PF08264: Anticodon_1" amino acids 718 to 838 (121 residues), 54.7 bits, see alignment E=2.8e-18

Best Hits

Swiss-Prot: 75% identical to SYL_BURCJ: Leucine--tRNA ligase (leuS) from Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 75% identity to bcj:BCAL3373)

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4Y682 at UniProt or InterPro

Protein Sequence (878 amino acids)

>RR42_RS17545 leucyl-tRNA synthetase (Cupriavidus basilensis FW507-4G11)
MQDKYLPSAVEQAAQQHWQAIDAYRVSEEAKLADGSEKPKFYACSMLPYPSGKLHMGHVR
NYTINDMMARHLRMNGHNVLMPMGWDAFGMPAENAALNNGVAPAAWTYNNIAEMKRQMQS
MGLAIDWSREVATCSPDYYRWNQWLFLKMLEKGIAYRKTGTVNWDPVDQTVLANEQVIDG
RGWRSGALVEKREIPMYYLRITDYAEELLGDLDDLGWPERVKIMQQNWIGKSVGVRFGFT
HAIAGDDGKLIGDGKLYVFTTRADTIMGVTFCAVAAEHPLATHAAATNPELAAFINECKH
GSVMEADMATMEKKGMPTGLKVVHPLTGEQVEVWVGNYVLMSYGDGAVMGVPAHDERDFA
FANKYGLPIKQVVDMVDAKKGQPFSTEAWQEWYGDKEHGTCIHSGKYDGLDYKAAVEAIA
ADLGAKGLGEKKITWRLRDWGISRQRYWGTPIPLIHCDGCGVVPVPEQDLPVVLPEDLVP
DGTGNPLAKDPRFLACTCPSCGKPARRETDTMDTFIDSCWYYMRYTCPDAATMVDARNDY
WMPMDQYIGGIEHAILHLLYARFWTKVMRDMGLVTFNEPFSNLLTQGMVLNDTYYREDAA
GKKTWYNPAEVDVSTDERGRPLGAVLKADGQPVVIGGVEKMSKSKNNGIDPQALIDQYGA
DTARLFVMFAAPPEQQLEWSGSGVEGASRFLRRVWNYGYANAEAIAAAGNAVPSGDADAA
LRREIHGVLKQANYDYQRIQYNTVVSATMKMLNALEDAKQATPAARREGLGILLRVLYPV
VPHITHGLWQELGYATQYGDLLDAAWPQVDESALVQSELDLVLQINGKVRGSLKVPAVAD
RAAIEAMAAASEIVARFAEGAAPKKIVVVPGRLVNVVL