Protein Info for RR42_RS17390 in Cupriavidus basilensis FW507-4G11

Annotation: ATP-dependent DNA helicase RecG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 733 PF17191: RecG_wedge" amino acids 48 to 193 (146 residues), 35.8 bits, see alignment E=1.5e-12 TIGR00643: ATP-dependent DNA helicase RecG" amino acids 57 to 705 (649 residues), 696.4 bits, see alignment E=2.2e-213 PF04851: ResIII" amino acids 297 to 471 (175 residues), 28.1 bits, see alignment E=4.4e-10 PF00270: DEAD" amino acids 301 to 476 (176 residues), 77.8 bits, see alignment E=2.1e-25 PF00271: Helicase_C" amino acids 520 to 627 (108 residues), 62.6 bits, see alignment E=9.8e-21 PF19833: RecG_dom3_C" amino acids 656 to 716 (61 residues), 39.6 bits, see alignment 1.2e-13

Best Hits

KEGG orthology group: K03655, ATP-dependent DNA helicase RecG [EC: 3.6.4.12] (inferred from 83% identity to reh:H16_A3111)

Predicted SEED Role

"ATP-dependent DNA helicase RecG (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YJN2 at UniProt or InterPro

Protein Sequence (733 amino acids)

>RR42_RS17390 ATP-dependent DNA helicase RecG (Cupriavidus basilensis FW507-4G11)
MPGTAPDRTPETNDSPPAARAGDKPGRAGKAAAKGAAAGKPSSAMARLHKLGLKRDVDLV
LHLPMRYEDETTLLPIADAIGRAGMGLTVQVEGVVTSNEVSLRPRRQMVVKIADDTGELT
LRFINFYGSQAQLMAEGACLRVRGELRGGFFGAEMVHPTVRAVTPGEALPERLTPVYPST
AGIPQSYLRKAIGGALSRTPLPETLPVPVLQGPLAQLKLQALPECLRLLHTPPQDVDEHA
LIERSHPAWQRIKLDELLAQQLSLKRSQAARRDKSAPPMPRRAGGLLTAFLAALPFKLTG
AQQRVVEEIARDMGAPHPMHRLLQGDVGSGKTIVAALAACQAIDAGYQAALMAPTEILAE
QHYRKLSAWLEPLGVPVVWLAGSLKTRAKREAAAKVESGEAKLAIGTHALIQDSVKFARL
GLSVVDEQHRFGVAQRLALRGKADPDATAAQGKAGKAAQTVPHQLMMSATPIPRTLAMTY
YADLDVSVIDELPPGRTPVVTRLVNDARRDEVIARVHHAAADGRQVYWVCPLIEESEALQ
LQTAVETYETLVAALPELKVGLVHGRLPPAEKAAVMEDFTANRLQVLVATTVIEVGVDVP
NASLMVIEHAERFGLAQLHQLRGRVGRGSAESVCLLMYQAPLSPTGRERLATMRETTDGF
EIARRDLQIRGPGEFLGARQSGEAMLRFADLNTDAWLVDYAQEAAELMLARFPEAVEAHL
LRWLGGREHYLKA