Protein Info for RR42_RS17315 in Cupriavidus basilensis FW507-4G11

Updated annotation (from data): D-lactate dehydrogenase, iron-sulfur subunit GlcF (EC 1.1.99.6)
Rationale: Important on D-lactate
Original annotation: glycolate oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 421 PF13237: Fer4_10" amino acids 23 to 86 (64 residues), 31.1 bits, see alignment E=6.6e-11 PF13183: Fer4_8" amino acids 23 to 89 (67 residues), 45.7 bits, see alignment E=2.8e-15 PF12838: Fer4_7" amino acids 24 to 89 (66 residues), 42.5 bits, see alignment E=2.5e-14 PF13534: Fer4_17" amino acids 24 to 89 (66 residues), 41.3 bits, see alignment E=6.3e-14 PF02754: CCG" amino acids 176 to 260 (85 residues), 51.5 bits, see alignment E=3.2e-17 amino acids 318 to 401 (84 residues), 49.8 bits, see alignment E=1.1e-16

Best Hits

KEGG orthology group: K11473, glycolate oxidase iron-sulfur subunit (inferred from 79% identity to cti:RALTA_A2572)

Predicted SEED Role

"Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.99.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.14, 1.1.99.6

Use Curated BLAST to search for 1.1.99.14 or 1.1.99.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YDN4 at UniProt or InterPro

Protein Sequence (421 amino acids)

>RR42_RS17315 D-lactate dehydrogenase, iron-sulfur subunit GlcF (EC 1.1.99.6) (Cupriavidus basilensis FW507-4G11)
MQTTLAEFLRDTPDGEEAKSIVGKCVHCGFCTATCPTYQLLGDELDGPRGRIYLMKQVLE
GQPVTQSTRLHLDRCLTCRNCESTCPSGVKYGRLVDIGRKVVDDRLEAQGIQRPARERFA
RWALRETMTRPALFGTAMRMGQRVRPLLPQALRNKVPQAVDAGAWPRTTHARKMLLLDGC
VQPSMSPNINAATARVFDRLGVQLVMAREAGCCGAVRYHTGDHDGGLDNMRRNIDAWWPA
VQAGAEAIVMTASGCGVMVKEYGHLLRNDAHYADRARQISALTKDLSELLPNFADALQDA
AAEAGSSKGTNGTDGQRVAYHPPCTLQHGQQIRGKVEALLTGLGVDVKLCADSHLCCGSA
GTYSVLQPALSYRLRDEKLANLQALKPEAIVSANIGCITHLQSGTGTPVMHWIELVDRML
G