Protein Info for RR42_RS16905 in Cupriavidus basilensis FW507-4G11

Updated annotation (from data): Imidazolonepropionase (EC 3.5.2.7)
Rationale: Specifically important for utilizing L-Histidine. Automated validation from mutant phenotype: the predicted function (IMIDAZOLONEPROPIONASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: imidazolonepropionase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01224: imidazolonepropionase
  • amino acids 31 to 408 (378 residues), 484.4 bits, E=1e-149
• PF01979: Amidohydro_1
  • amino acids 67 to 408 (342 residues), 61.9 bits, E=6.5e-21
• PF07969: Amidohydro_3
  • amino acids 117 to 385 (269 residues), 45 bits, E=1.2e-15

Best Hits

Swiss-Prot: 84% identical to HUTI_CUPNJ: Imidazolonepropionase (hutI) from Cupriavidus necator (strain JMP 134 / LMG 1197)

KEGG orthology group: K01468, imidazolonepropionase [EC: 3.5.2.7] (inferred from 84% identity to reu:Reut_A2713)

MetaCyc: 65% identical to imidazolonepropionase (Pseudomonas fluorescens)
Imidazolonepropionase. [EC: 3.5.2.7]

Predicted SEED Role

"Imidazolonepropionase (EC 3.5.2.7)" in subsystem Histidine Degradation (EC 3.5.2.7)

MetaCyc Pathways

• L-histidine degradation II (5/5 steps found)
• L-histidine degradation VI (7/8 steps found)
• L-histidine degradation I (4/4 steps found)
• L-histidine degradation III (4/6 steps found)

KEGG Metabolic Maps

• Histidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.2.7

Sequence Analysis Tools

See A0A0C4Y5X0 at UniProt or InterPro

Protein Sequence (420 amino acids)

>RR42_RS16905 Imidazolonepropionase (EC 3.5.2.7) (Cupriavidus basilensis FW507-4G11)
MSSSTESAKSADGVWHRCHLLPDGDPAKAIRDAALVVQQGRIVWLGMQAELPQAYAALPR
HDAGSAWITPGLVDCHTHLVYGGQRADEFAMRLAGAGYEEIARAGGGIVSTVRATRAADE
DTLFALAAARLAPLLAEGVTTLEIKSGYGLDLASERKQLRVARRLGDAFGVTVHTTFLGA
HALPPEYAGRADDYITLVCETMMPVLADEGLIDAVDAFCESVGFSLAQTERVFQAAERHG
LRVKLHAEQLSNLGGTALAARYRALSADHLEHLDEAGVVAMAGSGTVAVLLPGAYYFLRD
TNLPPIDLLRRHGVPMAISTDHNPGTSPVTSLLLMMNMACTLFRLTVPEVLAGVTTHAAR
ALGAADRHGLLAAGRAADFVLWNVDSPAELAYWFGRNPAAAVVRQGKVYPAATSVQGVRP