Protein Info for RR42_RS15595 in Cupriavidus basilensis FW507-4G11

Annotation: acetoacetyl-CoA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 678 transmembrane" amino acids 159 to 174 (16 residues), see Phobius details TIGR01217: acetoacetate-CoA ligase" amino acids 6 to 663 (658 residues), 782.7 bits, see alignment E=1.2e-239 PF16177: ACAS_N" amino acids 41 to 95 (55 residues), 47.2 bits, see alignment 1.5e-16 PF00501: AMP-binding" amino acids 108 to 483 (376 residues), 150.8 bits, see alignment E=5.1e-48

Best Hits

KEGG orthology group: K01907, acetoacetyl-CoA synthetase [EC: 6.2.1.16] (inferred from 84% identity to cti:RALTA_A2344)

Predicted SEED Role

"Acetoacetyl-CoA synthetase (EC 6.2.1.16)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Polyhydroxybutyrate metabolism (EC 6.2.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.16

Use Curated BLAST to search for 6.2.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YEU8 at UniProt or InterPro

Protein Sequence (678 amino acids)

>RR42_RS15595 acetoacetyl-CoA synthetase (Cupriavidus basilensis FW507-4G11)
MTTLEEGQLRWTPSQAFRDGSRIAHFMRWLESERGLAFADYGALWQWSVTDLEAFWDAIR
AYFDLRFDTPAERVLGSAQMPGARWFEGASLNYVQQVFRHAGSGAARERTAIRYAGESIA
VTDLSWDVLEQQVASLAHALRGMGVARGDRVAGYLPNIPATVVAFLATASLGAIWSGCAP
DMGQVAVSDRFRQIEPKVLIAVDGYRYGGKAYDRAPVLADLAAALPSLTDLVLVPGEHTD
AHAAPNAIALPANVRRHAWQAVLAHRVPLAIESVPFDHPLWIVYSSGTTGMPKPIVHGHG
GIVIEQLKLMAFHNNLGPDDVFHWYSSSGWIMWNAQIAGLLLGSTIALYDGNPAWPDAGV
LWRFVDAARVTAFGAGAAFFTAGMKAGIEPARVADLSRLRALGSTGSPLPAEAYDWIYRH
VSADIWLAPMSGGTDFAGSFVAGCPILPVYSGEMQCRCLGAKVEAFDEAGNALVGEVGEL
VCTAPMPSMPLFLWGDTNGQRYRDSYFDTYPGVWRHGDWIKITAHGGAIIYGRSDATINR
HGIRMGTSELYRVVEDLPEVLDSMVVDLEYLGRDSYMPLFVVLREGMVLDDALRDTMRAR
IRSALSSRHVPNEILQVPGVPRTLSGKKMEVPIKKLLLGHAPDKIANRDAMANPETLDWY
FAYAERFIAARASESAAA