Protein Info for RR42_RS15485 in Cupriavidus basilensis FW507-4G11

Annotation: serine hydroxymethyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00464: SHMT
  • amino acids 10 to 383 (374 residues), 560.1 bits, E=4.4e-172
• PF00155: Aminotran_1_2
  • amino acids 80 to 364 (285 residues), 42.8 bits, E=8.1e-15
• PF01212: Beta_elim_lyase
  • amino acids 81 to 343 (263 residues), 28 bits, E=2.7e-10
• PF00266: Aminotran_5
  • amino acids 81 to 251 (171 residues), 23.6 bits, E=4.8e-09

Best Hits

Swiss-Prot: 94% identical to GLYA_CUPNH: Serine hydroxymethyltransferase (glyA) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K00600, glycine hydroxymethyltransferase [EC: 2.1.2.1] (inferred from 93% identity to cti:RALTA_A2322)

MetaCyc: 64% identical to serine hydroxymethyltransferase (Escherichia coli K-12 substr. MG1655)
4.1.2.-; Glycine hydroxymethyltransferase. [EC: 2.1.2.1]; RXN-6321 [EC: 2.1.2.1]; RXN0-5240 [EC: 2.1.2.1]

Predicted SEED Role

"Serine hydroxymethyltransferase (EC 2.1.2.1)" in subsystem Folate Biosynthesis or Glycine Biosynthesis or Glycine and Serine Utilization or LMPTP YwlE cluster or Photorespiration (oxidative C2 cycle) or Serine-glyoxylate cycle or Serine Biosynthesis (EC 2.1.2.1)

MetaCyc Pathways

• folate transformations III (E. coli) (9/9 steps found)
• folate transformations II (plants) (10/11 steps found)
• superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
• dTMP de novo biosynthesis (mitochondrial) (3/3 steps found)
• glycine degradation (3/3 steps found)
• formaldehyde assimilation I (serine pathway) (10/13 steps found)
• folate polyglutamylation (4/5 steps found)
• glycine biosynthesis I (1/1 steps found)
• folate transformations I (9/13 steps found)
• photorespiration I (6/9 steps found)
• photorespiration III (6/9 steps found)
• glycine betaine degradation I (5/8 steps found)
• glycine betaine degradation III (4/7 steps found)
• photorespiration II (6/10 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• glycine betaine degradation II (mammalian) (1/4 steps found)

KEGG Metabolic Maps

• Cyanoamino acid metabolism
• Glycine, serine and threonine metabolism
• Methane metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.2.1

Sequence Analysis Tools

See A0A0C4Y553 at UniProt or InterPro

Protein Sequence (415 amino acids)

>RR42_RS15485 serine hydroxymethyltransferase (Cupriavidus basilensis FW507-4G11)
MFERSRYTIDQIDPELFAAIQKEDQRQEEHIELIASENYTSPAVMAAQGSQLTNKYAEGY
PGKRYYGGCEYVDIVEQLAIDRVKQLFGAEAANVQPNSGSQANQGVFFAALKPGDTIMGM
SLAEGGHLTHGMALNMSGKWFNVVSYGLDANEDIDYDALEKLAQEKKPKLIIAGASAFAL
RIDFERISKVAKSIGALFMVDMAHYAGLIAAGEYPNPVPHADFVTTTTHKSLRGPRGGVI
LMKAEHEKAINSAIFPGIQGGPLMHVIAGKAVAFKEALSPEFKTYQQQVVKNARVLAETL
IERGLRIVSGRTESHVMLVDLRAMQITGKEAEKVLGEAHITVNKNAIPNDPEKPFVTSGI
RLGSPAMTTRGFKEEEARLVANLVADVLENPHDLANIARVREQVAALTKRFPVYG