Protein Info for RR42_RS15385 in Cupriavidus basilensis FW507-4G11

Updated annotation (from data): Kynureninase (EC 3.7.1.3)
Rationale: Specifically important for utilizing L-Tryptophan. Automated validation from mutant phenotype: the predicted function (KYNURENINASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: kynureninase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01814: kynureninase
  • amino acids 10 to 402 (393 residues), 355.4 bits, E=1.9e-110
• PF00266: Aminotran_5
  • amino acids 81 to 353 (273 residues), 58.2 bits, E=7.5e-20
• PF01041: DegT_DnrJ_EryC1
  • amino acids 106 to 210 (105 residues), 22.4 bits, E=6.7e-09

Best Hits

Swiss-Prot: 72% identical to KYNU_PSEFL: Kynureninase (kynU) from Pseudomonas fluorescens

KEGG orthology group: K01556, kynureninase [EC: 3.7.1.3] (inferred from 88% identity to reu:Reut_A0808)

MetaCyc: 72% identical to kynureninase subunit (Pseudomonas fluorescens)
Kynureninase. [EC: 3.7.1.3]

Predicted SEED Role

"Kynureninase (EC 3.7.1.3)" in subsystem NAD and NADP cofactor biosynthesis global (EC 3.7.1.3)

MetaCyc Pathways

• L-tryptophan degradation IX (11/12 steps found)
• L-tryptophan degradation XII (Geobacillus) (11/12 steps found)
• NAD de novo biosynthesis II (from tryptophan) (8/9 steps found)
• superpathway of NAD biosynthesis in eukaryotes (11/14 steps found)
• L-tryptophan degradation I (via anthranilate) (3/3 steps found)
• L-tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde (4/5 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (25/35 steps found)
• 3-hydroxy-4-methyl-anthranilate biosynthesis II (3/5 steps found)
• L-tryptophan degradation III (eukaryotic) (10/15 steps found)
• L-tryptophan degradation XI (mammalian, via kynurenine) (11/23 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (21/42 steps found)

KEGG Metabolic Maps

• Tryptophan metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.7.1.3

Sequence Analysis Tools

See A0A0C4YID7 at UniProt or InterPro

Protein Sequence (418 amino acids)

>RR42_RS15385 Kynureninase (EC 3.7.1.3) (Cupriavidus basilensis FW507-4G11)
MTAMTREQCLALDQEDPLRRLRDQFALPQGVIYLDGNSLGARPRSAAARAAEVVSEEWGT
GLIRSWNTAGWFELPQRLGNMLAPLIGAGEDEVVVTDTTSSNLFKVLAAALRVQQTRDPK
RKVIVSEASNFPTDLYIAQGLADLLQQGYSLRLVNAPEEIDAAVDADTAVLMLTHVNYKT
GEMLDMASVTEMAHARGALTVWDLAHSAGAVPVALKASGADYAVGCTYKYLNGGPGSPAF
LWVAPSLRDAFWQPLSGWWGHAAPFAMEPGYRPREGVARFLCGTQPIASLAMVECGLDIY
AQTDMAVLRAKSLMLTDLFIALVETRCAAHPLELVTPRAHARRGSHVSFAHPDGFAVVQA
LIERGVIGDYREPRIARFGFTPLYTSFTEVWDAVEILRDVLDSGTYKAERHQTRGLVT