Protein Info for RR42_RS15150 in Cupriavidus basilensis FW507-4G11

Annotation: alanyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01411: tRNA-synt_2c
  • amino acids 5 to 556 (552 residues), 727.1 bits, E=2.1e-222
• TIGR00344: alanine--tRNA ligase
  • amino acids 5 to 853 (849 residues), 1050.2 bits, E=0
• PF07973: tRNA_SAD
  • amino acids 653 to 695 (43 residues), 57 bits, 2.3e-19
• PF02272: DHHA1
  • amino acids 733 to 870 (138 residues), 102.2 bits, E=5e-33

Best Hits

Swiss-Prot: 91% identical to SYA_CUPNH: Alanine--tRNA ligase (alaS) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 81% identity to bph:Bphy_1613)

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

• tRNA charging (20/21 steps found)
• superpathway of chorismate metabolism (45/59 steps found)
• shinorine biosynthesis (3/8 steps found)
• vanchrobactin biosynthesis (2/8 steps found)
• enterobactin biosynthesis (4/11 steps found)
• ergotamine biosynthesis (4/12 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• bacillibactin biosynthesis (3/12 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (4/25 steps found)
• phosphinothricin tripeptide biosynthesis (4/25 steps found)
• corallopyronin A biosynthesis (2/30 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.7

Sequence Analysis Tools

See A0A0C4Y4Y3 at UniProt or InterPro

Protein Sequence (874 amino acids)

>RR42_RS15150 alanyl-tRNA synthetase (Cupriavidus basilensis FW507-4G11)
MKVSDIRSKFLQYFESKGHTVVRSSSLVPGNDPTLLFTNSGMVQFKDVFLGTDKRPYTRA
TSSQRSVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKREAIQYAWELLTKVYQLP
AEKLWVTVYAEDDEAYDIWAKEVGVPPERIVRIGDNKGARYASDNFWQMADTGPCGPCSE
IFYDHGPDVWGGPPGSPEEDGDRYIEIWNLVFMQFNRDEQGNMPRLPKPCVDTGMGLERI
AAVLQHVHSNYEIDLFQALIKGAARETGTTDLNNNSLKVIADHIRACSFLIVDGVIPGNE
GRGYVLRRIIRRAIRHGYKLGRKTPFFHKLVGDLVGQMGQAYPELSDAEARVVEVLKAEE
ERFFETIENGMSILDAALADVKAKGGTVLDGELAFKLHDTFGFPLDLTQDVCREQEMTVD
EAAFDAAMSRQREQARAAGKFKMAAGLEYKGDKTTFHGYEKLGVDGAKITALYVDGASVD
TMQPGQTGVIVLDNTPFYAESGGQAGDQGELKSGSAVFSVVDTTKVQADVFGHQGTLAGG
ALKVGDSVTAQVDAQRRARTVRNHSATHLMHKALREVLGSHVQQKGSLVDADKTRFDFSH
NAPMTDEQIRRVEEIVNAEILQNAATGAEVMPFDDAVKSGAMALFGEKYADDVRVLSIGS
SKELCGGTHVTRTGDIGVFKIVVESGVAAGIRRVEAITGDNALHYLQALDAKLNEAAAAL
KAQPSELVPRIGQVQEQVRALEKELERLKSKLAASQGDELAAQAIDVKGIKVLAAQLDGA
DVKTLRETMDKLKDKLGTAAIVLGAVADGKVSLIAGVTADATAKVKAGELVNFVAQQVGG
KGGGRPDMAQAGGTEPANLPQALAGVGEWVASKV